| HEJÁTKO, Jan, P. BREWER, M. HEISLER, Jiří FRIML a Eva BENKOVÁ. In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature Protocols. U.S.A.: Nature Publishing Group, 2006, roč. 1, č. 3, s. 1939 - 1946, 6 s. ISSN 1745-2473. |
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@article{707319,
author = {Hejátko, Jan and Brewer, P. and Heisler, M. and Friml, Jiří and Benková, Eva},
article_location = {U.S.A.},
article_number = {3},
keywords = {in situ mRNA localization; tissue sections; plants},
language = {eng},
issn = {1745-2473},
journal = {Nature Protocols},
title = {In situ hybridization for mRNA detection in Arabidopsis tissue sections.},
volume = {1},
year = {2006}
}
TY - JOUR
ID - 707319
AU - Hejátko, Jan - Brewer, P. - Heisler, M. - Friml, Jiří - Benková, Eva
PY - 2006
TI - In situ hybridization for mRNA detection in Arabidopsis tissue sections.
JF - Nature Protocols
VL - 1
IS - 3
SP - 1939 - 1946
EP - 1939 - 1946
PB - Nature Publishing Group
SN - 17452473
KW - in situ mRNA localization
KW - tissue sections
KW - plants
N2 - Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days.
ER -
HEJÁTKO, Jan, P. BREWER, M. HEISLER, Jiří FRIML a Eva BENKOVÁ. In situ hybridization for mRNA detection in Arabidopsis tissue sections. \textit{Nature Protocols}. U.S.A.: Nature Publishing Group, 2006, roč.~1, č.~3, s.~1939 - 1946, 6 s. ISSN~1745-2473.
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