2006
Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins
BITTOVÁ, Miroslava; Jiří PAZOUREK a Josef HAVELZákladní údaje
Originální název
Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins
Název česky
Electroosmotic Flow Changes Due to Interactions of Background Electrolyte with Polyethyleneimine Coating in Capillary Zone Electrophoresis of Proteins
Autoři
Vydání
Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2006, 1615-9306
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.535
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/06:00016303
Organizační jednotka
Přírodovědecká fakulta
UT WoS
Klíčová slova anglicky
polyethyleneimine; capillary coating; electroosmotic flow; protein adsorption
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 29. 4. 2011 09:06, Mgr. Miroslava Bittová, Ph.D.
V originále
The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge in the capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. As a model sample, a standard protein mixture of cytochrome c, ribonuclease A and lysozyme in concentration of 0.25 mg/ml each was chosen.
Česky
The properties and behavior of polyethyleneimine (PEI) covalently coated capillaries with respect to different background electrolytes used in capillary zone electrophoresis (CZE) is described. The coating stability and changes of inner surface charge in the capillary were followed by electroosmotic flow (EOF) measurement. The main interest was focused onto conjugate bases of carboxylic acids as anionic background electrolyte components (acetate, citrate, malate, malonate, tartrate and succinate). Interesting phenomenon in PEI coated capillaries was observed: the direction (and the magnitude) of EOF depends on the composition of the background electrolyte and at a certain pH it can reversibly change. To explain this behavior, a hypothesis of an ionic complex formation was suggested. With this knowledge, the PEI coated capillary was used for the separation of basic proteins in mentioned background electrolytes. As a model sample, a standard protein mixture of cytochrome c, ribonuclease A and lysozyme in concentration of 0.25 mg/ml each was chosen.
Návaznosti
| GA305/03/1100, projekt VaV |
|