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@inproceedings{709265,
author = {Španová, Alena and Rittich, Bohuslav and Horák, Daniel and Rybnikář, Alois},
address = {Krems},
booktitle = {6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers},
keywords = {Magnetic carriers; DNA; Dairy products; PCR},
language = {eng},
location = {Krems},
pages = {196-196},
publisher = {University of Applied Sciences Krems, Austria},
title = {Hydrophilic magnetic particles for molecular diagnostics},
year = {2006}
}
TY - JOUR
ID - 709265
AU - Španová, Alena - Rittich, Bohuslav - Horák, Daniel - Rybnikář, Alois
PY - 2006
TI - Hydrophilic magnetic particles for molecular diagnostics
PB - University of Applied Sciences Krems, Austria
CY - Krems
KW - Magnetic carriers
KW - DNA
KW - Dairy products
KW - PCR
N2 - Magnetic nonporous P(HEMA-co-EDMA) particles were prepared by dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) in the presence of maghemite nanoparticles. Magnetic nonporous P(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA) in the presence of colloidal oleic acid-coated magnetite (Fe3O4) particles. Subsequently, the hydroxy groups of the particles were oxidized with 2 wt.% aqueous solution of potassium permanganate under acidic conditions (2 M sulphuric acid). Prepared particles were used for isolation of bacterial DNA from real samples. DNA was adsorbed on particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations. DNA was eluted using TE buffer and used directly for PCR amplification and identification Lactobacillus and Bifidobacterium cells in dairy products, Trichophyton cells in lyophilisates, bacterial cells in sediments from Lake Baikal.
ER -
ŠPANOVÁ, Alena, Bohuslav RITTICH, Daniel HORÁK a Alois RYBNIKÁŘ. Hydrophilic magnetic particles for molecular diagnostics. In \textit{6th International Conference on the Scietific and Clinical applicationsof magnetic Carriers}. Krems: University of Applied Sciences Krems, Austria, 2006, s.~196-196.
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