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@proceedings{709493,
author = {Nováková, Dana and Švec, Pavel and Sedláček, Ivo},
booktitle = {2nd FEMS Congress of European Microbiologists},
keywords = {staphylococcal type strains; whole-cell protein analysis; (GTG)5-PCR fingerprinting},
language = {eng},
title = {Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.},
year = {2006}
}
TY - CONF
ID - 709493
AU - Nováková, Dana - Švec, Pavel - Sedláček, Ivo
PY - 2006
TI - Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.
KW - staphylococcal type strains
KW - whole-cell protein analysis
KW - (GTG)5-PCR fingerprinting
N2 - A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecies were separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed. However, most of the phylogenetically closely related coagulase-negative novobiocin-resistant species revealed very similar protein patterns. Our results showed the whole-cell protein profile analysis as well as rep-PCR fingerprinting with the (GTG)5 primer as suitable typing tools for characterization of staphylococci and their combination could be a helpful approach for identification of staphylococci. Financial support from project FRVS 2658 is gratefully acknowledged.
ER -
NOVÁKOVÁ, Dana, Pavel ŠVEC a Ivo SEDLÁČEK. Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting. In \textit{2nd FEMS Congress of European Microbiologists}. 2006.
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