2006
Shape-specific recognition in the structure of the Vts1p SAM domain with RNA
OBERSTRASS, Florian, Albert LEE, Richard ŠTEFL, Michael JANIS, Guillaume CHANFREAU et. al.Základní údaje
Originální název
Shape-specific recognition in the structure of the Vts1p SAM domain with RNA
Název česky
Shape-specific recognition in the structure of the Vts1p SAM domain with RNA
Autoři
OBERSTRASS, Florian (756 Švýcarsko), Albert LEE (840 Spojené státy), Richard ŠTEFL (203 Česká republika, garant), Michael JANIS (840 Spojené státy), Guillaume CHANFREAU (250 Francie) a Frederic ALLAIN (250 Francie)
Vydání
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 1545-9985
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 11.502
Kód RIV
RIV/00216224:14310/06:00018474
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000235160500015
Klíčová slova anglicky
SACCHAROMYCES-CEREVISIAE; REPRESSES TRANSLATION; DEADENYLASE COMPLEX; DROSOPHILA EMBRYO; DIPOLAR COUPLINGS; CRYSTAL-STRUCTURE; CCR4-NOT COMPLEX; GENE-EXPRESSION
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 22. 2. 2007 13:16, prof. Mgr. Richard Štefl, Ph.D.
V originále
Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE.
Česky
Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE.
Návaznosti
| MSM0021622413, záměr |
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