Using of liquid chromatography coupled with diode array
detector for determination of naphthoquinones in plants and for
investigation of influence of pH of cultivation medium on
content of plumbagin in Dionaea muscipula

J 2006

Using of liquid chromatography coupled with diode array detector for determination of naphthoquinones in plants and for investigation of influence of pH of cultivation medium on content of plumbagin in Dionaea muscipula

BABULA, Petr, Radka MIKELOVÁ, Vojtech ADAM, René KIZEK, L. HAVEL et. al.

Základní údaje

Originální název

Using of liquid chromatography coupled with diode array detector for determination of naphthoquinones in plants and for investigation of influence of pH of cultivation medium on content of plumbagin in Dionaea muscipula

Název česky

Využití kapalinové chromatografie ve spojení s diod array detektorem pro stanovení naftochinonů v rostlinách a pro zkoumání vlivu pH kultivačního média na množství plumbaginu v Dionaea muscipula

Autoři

BABULA, Petr (203 Česká republika), Radka MIKELOVÁ (203 Česká republika, garant), Vojtech ADAM (203 Česká republika), René KIZEK (203 Česká republika), L. HAVEL (203 Česká republika) a Z. SLADKÝ (203 Česká republika)

Vydání

Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2006, 1570-0232

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.647

Kód RIV

RIV/00216224:14310/06:00016393

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000240712700005

Klíčová slova anglicky

liquid chromatography coupled with diode array detector; naphthoquinones; plumbagin; Dionaea muscipula

Štítky

Dionaea muscipula, naphthoquinones, Plumbagin

Změněno: 14. 2. 2007 17:55, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

The interest of many investigators in naphthoquinones is due to their broad-range of biological actions from phytotoxic to fungicidal. The main aim of this work was to investigate the influence of different pH values of cultivation medium on naphthoquinone content in Dionaea muscipula. For this purpose, we optimized the simultaneous analysis of the most commonly occurring naphthoquinones (1,4-naphthoquinone, lawsone, juglone and plumbagin) by high performance liquid chromatography coupled with diode array detector (HPLC-DAD). The most suitable chromatographic conditions were as follows: mobile phase: 0.1 mol l(-1) acetic acid:methanol in ratio of 33:67 (%, v/v), flow rate: 0.75 ml min(-1) and temperature: 42 degrees C., Moreover, we looked for the most suitable technique for preparation of plant samples (D. muscipula, Juglans regia, Paulownia tomentosa, Impatience glandulifera, Impatience parviflora, Drosera rotundifolia, Drosera spathulata and Drosera capensis) due to their consequent analysis by HPLC-DAD. It clearly follows from the results obtained that sonication were the most suitable technique for preparation of J. regia plants. We also checked the recoveries of the determined naphthoquinones, which were from 96 to 104%. Finally, we investigated the changes in content of plumbagin in D. muscipula plants according to different pH of cultivation medium. The content increased with increasing pH up to 5 and, then, changed gradually. The lower content of plumbagin at lower pH values was of interest to us.

Česky

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

Citovat

BABULA, Petr, Radka MIKELOVÁ, Vojtech ADAM, René KIZEK, L. HAVEL a Z. SLADKÝ. Using of liquid chromatography coupled with diode array detector for determination of naphthoquinones in plants and for investigation of influence of pH of cultivation medium on content of plumbagin in Dionaea muscipula. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2006, roč. 842, č. 1, s. 28-35, 7 s. ISSN 1570-0232.

@article{712562,
   author = {Babula, Petr and Mikelová, Radka and Adam, Vojtech and Kizek, René and Havel, L. and Sladký, Z.},
   article_location = {Amsterdam, The Netherlands},
   article_number = {1},
   keywords = {liquid chromatography coupled with diode array detector; naphthoquinones; plumbagin; Dionaea muscipula},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B},
   title = {Using of liquid chromatography coupled with diode array detector for determination of naphthoquinones in plants and for investigation of influence of pH of cultivation medium on content of plumbagin in Dionaea muscipula},
   volume = {842},
   year = {2006}
}

TY  - JOUR
ID  - 712562
AU  - Babula, Petr - Mikelová, Radka - Adam, Vojtech - Kizek, René - Havel, L. - Sladký, Z.
PY  - 2006
TI  - Using of liquid chromatography coupled with diode array detector for determination of naphthoquinones in plants and for investigation of influence of pH of cultivation medium on content of plumbagin in Dionaea muscipula
JF  - Journal of Chromatography B
VL  - 842
IS  - 1
SP  - 28-35
EP  - 28-35
PB  - Elsevier
SN  - 15700232
KW  - liquid chromatography coupled with diode array detector
KW  - naphthoquinones
KW  - plumbagin
KW  - Dionaea muscipula
N2  - The interest of many investigators in naphthoquinones is due to their broad-range of biological actions from phytotoxic to fungicidal. The main aim of this work was to investigate the influence of different pH values of cultivation medium on naphthoquinone content in Dionaea muscipula. For this purpose, we optimized the simultaneous analysis of the most commonly occurring naphthoquinones (1,4-naphthoquinone, lawsone, juglone and plumbagin) by high performance liquid chromatography coupled with diode array detector (HPLC-DAD). The most suitable chromatographic conditions were as follows: mobile phase: 0.1 mol l(-1) acetic acid:methanol in ratio of 33:67 (%, v/v), flow rate: 0.75 ml min(-1) and temperature: 42 degrees C., Moreover, we looked for the most suitable technique for preparation of plant samples (D. muscipula, Juglans regia, Paulownia tomentosa, Impatience glandulifera, Impatience parviflora, Drosera rotundifolia, Drosera spathulata and Drosera capensis) due to their consequent analysis by HPLC-DAD. It clearly follows from the results obtained that sonication were the most suitable technique for preparation of J. regia plants. We also checked the recoveries of the determined naphthoquinones, which were from 96 to 104%. Finally, we investigated the changes in content of plumbagin in D. muscipula plants according to different pH of cultivation medium. The content increased with increasing pH up to 5 and, then, changed gradually. The lower content of plumbagin at lower pH values was of interest to us.
ER  -

BABULA, Petr, Radka MIKELOVÁ, Vojtech ADAM, René KIZEK, L. HAVEL a Z. SLADKÝ. Using of liquid chromatography coupled with diode array detector for determination of naphthoquinones in plants and for investigation of influence of pH of cultivation medium on content of plumbagin in Dionaea muscipula. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2006, roč.~842, č.~1, s.~28-35, 7 s. ISSN~1570-0232.

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