GFP assay as a sensitive eukaryotic screening model to detect
toxic and genotoxic activity of azaarenes.

J 2006

GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes.

BARTOŠ, Tomáš; Štefan LETZSCH; Michal ŠKAREK; Zuzana FLEGROVÁ; Pavel ČUPR et al.

Základní údaje

Originální název

GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes.

Název česky

Test GFP jako citlivý screeningový model k detekci toxicity a genotoxické aktivity azaarenů

Autoři

BARTOŠ, Tomáš; Štefan LETZSCH; Michal ŠKAREK; Zuzana FLEGROVÁ; Pavel ČUPR a Ivan HOLOUBEK

Vydání

Environmental Toxicology, USA, 2006, 1520-4081

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.582

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/06:00018644

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000239364500009

Klíčová slova anglicky

azaarenes; GFP yeast bioassay; genotoxicity; toxicity

Štítky

azaarenes, genotoxicity, GFP yeast bioassay, toxicity

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 3. 2010 22:49, doc. RNDr. Pavel Čupr, Ph.D.

Anotace

ORIG CZ

V originále

Azaarenes are nitrogen-containing polyaromatic heterocyclic compounds (NPAHs). The majority of the azaarenes found in the environment originate from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than polycyclic aromatic hydrocarbons (PAHs) concentrations, yet their biological effects can be of similar magnitude.Azaarenes are nitrogen-containing polyaromatic heterocyclic compounds (NPAHs). The majority of the azaarenes found in the environment originate from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than polycyclic aromatic hydrocarbons (PAHs) concentrations, yet their biological effects can be of similar magnitude. To assess the toxic and genotoxic activity, a green fluorescent protein (GFP) assay based on the yeast Saccharomyces cerevisiae was selected. In this study, the authors present evidence that genotoxicity assessment using the yeast GFP assay can provide a simple system to monitor the activity of these environmental pollutants that could possess mutagenic potential at low concentrations.

Česky

Test GFP jako citlivý screeningový model k detekci toxicity a genotoxické aktivity azaarenů

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

BARTOŠ, Tomáš; Štefan LETZSCH; Michal ŠKAREK; Zuzana FLEGROVÁ; Pavel ČUPR a Ivan HOLOUBEK. GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes. Environmental Toxicology. USA, 2006, roč. 21, č. 1, s. 343-348. ISSN 1520-4081.

@article{715508,
   author = {Bartoš, Tomáš and Letzsch, Štefan and Škarek, Michal and Flegrová, Zuzana and Čupr, Pavel and Holoubek, Ivan},
   article_location = {USA},
   article_number = {1},
   keywords = {azaarenes; GFP yeast bioassay; genotoxicity; toxicity},
   language = {eng},
   issn = {1520-4081},
   journal = {Environmental Toxicology},
   title = {GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes.},
   volume = {21},
   year = {2006}
}

TY  - JOUR
ID  - 715508
AU  - Bartoš, Tomáš - Letzsch, Štefan - Škarek, Michal - Flegrová, Zuzana - Čupr, Pavel - Holoubek, Ivan
PY  - 2006
TI  - GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes.
JF  - Environmental Toxicology
VL  - 21
IS  - 1
SP  - 343-348
EP  - 343-348
SN  - 15204081
KW  - azaarenes
KW  - GFP yeast bioassay
KW  - genotoxicity
KW  - toxicity
N2  - Azaarenes are nitrogen-containing polyaromatic heterocyclic compounds (NPAHs). The majority of the azaarenes found in the environment originate from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than polycyclic aromatic hydrocarbons (PAHs) concentrations, yet their biological effects can be of similar magnitude.Azaarenes are nitrogen-containing polyaromatic heterocyclic compounds (NPAHs). The majority of the azaarenes found in the environment originate from anthropogenic sources. Concentrations of NPAHs found in the environment are reported to be one to two orders of magnitude lower than polycyclic aromatic hydrocarbons (PAHs) concentrations, yet their biological effects can be of similar magnitude. To assess the toxic and genotoxic activity, a green fluorescent protein (GFP) assay based on the yeast Saccharomyces cerevisiae was selected. In this study, the authors present evidence that genotoxicity assessment using the yeast GFP assay can provide a simple system to monitor the activity of these environmental pollutants that could possess mutagenic potential at low concentrations.
ER  -

BARTOŠ, Tomáš; Štefan LETZSCH; Michal ŠKAREK; Zuzana FLEGROVÁ; Pavel ČUPR a Ivan HOLOUBEK. GFP assay as a sensitive eukaryotic screening model to detect toxic and genotoxic activity of azaarenes. \textit{Environmental Toxicology}. USA, 2006, roč.~21, č.~1, s.~343-348. ISSN~1520-4081.

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