The mink as an animal model for Pseudomonas aeruginosa
adhesion: binding of the bacterial lectins (PA-IL and PA-IIL)
to neoglycoproteins and to sections of pancreas and lung
tissues from healthy mink

J 2007

The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink

KIRKEBY, Svend, Michaela WIMMEROVÁ, Dennis MOE a Axel K. HANSEN

Základní údaje

Originální název

The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink

Název česky

Morče jako živočišný model pro adhezi bakterie Pseudomonas aeruginosa: vazba bakteriálních lektinů (PA-IL a PA-IIL)

Autoři

KIRKEBY, Svend (208 Dánsko), Michaela WIMMEROVÁ (203 Česká republika, garant), Dennis MOE (208 Dánsko) a Axel K. HANSEN (208 Dánsko)

Vydání

Microbes and Infection, Paris, Elsevier, 2007, 1286-4579

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.523

Kód RIV

RIV/00216224:14310/07:00020257

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000247107600003

Klíčová slova anglicky

pseudomonas aeruginosa; lectin;cystic fibrosis

Štítky

cystic fibrosis, lectin, Pseudomonas aeruginosa

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 5. 2007 15:53, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

ORIG CZ

V originále

Lung infection with Pseudomonas aeruginosa, leading to chronic lung disease with impaired function, is the major course of morbidity and mortality among cystic fibrosis patients. The bacterium produces two lectins that bind to a-D-galactose (PA-IL) and L-fucose (PA-IIL), respectively, and lectinecarbohydrate interactions may be involved in microbial pathogenicity by creating bacterial adherence to epithelial and endothelial cells. An ideal animal model for P. aeruginosa infection has until now not been established, but the mink seems to be the only animal that has been reported to develop spontaneous P. aeruginosa infections in the airways. Since cystic fibrosis also severely may affect pancreatic function, we incubated sections from mink lungs and pancreas with a medium containing Pseudomonas lectins in order to detect in situ binding of the bacterial lectins. In the lungs, both lectins adhered to seromucinous glands located in the submucosa of the larger bronchi. Additionally, PA-IL reacted with the capillaries in the alveolar walls and with the small blood vessels forming the vasa vasorum around the larger vessels, while PAIIL marked the goblet cells in the bronchial surface epithelium. In the pancreas, both lectins bound to the epithelium in the excretory ducts, and additionally, PA-IL strongly stained the pancreatic capillaries while PA-IIL staining was noticed in the apical part of acinar cells in the exocrine part of the gland while no lectin reaction could be recorded in the endocrine cells. Judging from the results in the present paper the mink should be considered a suitable model to study P. aeruginosa adherence.

Česky

Morče jako živočišný model pro adhezi bakterie Pseudomonas aeruginosa: vazba bakteriálních lektinů (PA-IL a PA-IIL)

Návaznosti

GA303/06/0570, projekt VaV

Název: Strukturně-funkční studie lektinů a adhezinů patogenních mikroorganismů

Investor: Grantová agentura ČR, Strukturně-funkční studie lektinů a adhezinů patogenních mikroorganismů

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

KIRKEBY, Svend, Michaela WIMMEROVÁ, Dennis MOE a Axel K. HANSEN. The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink. Microbes and Infection. Paris: Elsevier, 2007, roč. 9, č. 5, s. 566-573. ISSN 1286-4579.

@article{718507,
   author = {Kirkeby, Svend and Wimmerová, Michaela and Moe, Dennis and Hansen, Axel K.},
   article_location = {Paris},
   article_number = {5},
   keywords = {pseudomonas aeruginosa; lectin;cystic fibrosis},
   language = {eng},
   issn = {1286-4579},
   journal = {Microbes and Infection},
   title = {The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink},
   volume = {9},
   year = {2007}
}

TY  - JOUR
ID  - 718507
AU  - Kirkeby, Svend - Wimmerová, Michaela - Moe, Dennis - Hansen, Axel K.
PY  - 2007
TI  - The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink
JF  - Microbes and Infection
VL  - 9
IS  - 5
SP  - 566-573
EP  - 566-573
PB  - Elsevier
SN  - 12864579
KW  - pseudomonas aeruginosa
KW  - lectin;cystic fibrosis
N2  - Lung infection with Pseudomonas aeruginosa, leading to chronic lung disease with impaired function, is the major course of morbidity and mortality among cystic fibrosis patients. The bacterium produces two lectins that bind to a-D-galactose (PA-IL) and L-fucose (PA-IIL), respectively, and lectinecarbohydrate interactions may be involved in microbial pathogenicity by creating bacterial adherence to epithelial and endothelial cells. An ideal animal model for P. aeruginosa infection has until now not been established, but the mink seems to be the only animal that has been reported to develop spontaneous P. aeruginosa infections in the airways. Since cystic fibrosis also severely may affect pancreatic function, we incubated sections from mink lungs and pancreas with a medium containing Pseudomonas lectins in order to detect in situ binding of the bacterial lectins. In the lungs, both lectins adhered to seromucinous glands located in the submucosa of the larger bronchi. Additionally, PA-IL reacted with the capillaries in the alveolar walls and with the small blood vessels forming the vasa vasorum around the larger vessels, while PAIIL marked the goblet cells in the bronchial surface epithelium. In the pancreas, both lectins bound to the epithelium in the excretory ducts, and additionally, PA-IL strongly stained the pancreatic capillaries while PA-IIL staining was noticed in the apical part of acinar cells in the exocrine part of the gland while no lectin reaction could be recorded in the endocrine cells. Judging from the results in the present paper the mink should be considered a suitable model to study P. aeruginosa adherence.
ER  -

KIRKEBY, Svend, Michaela WIMMEROVÁ, Dennis MOE a Axel K. HANSEN. The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink. \textit{Microbes and Infection}. Paris: Elsevier, 2007, roč.~9, č.~5, s.~566-573. ISSN~1286-4579.

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