Cell death induced by sodium nitroprusside and hydrogen
peroxide in tobacco BY 2 cell suspension

J 2007

Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY 2 cell suspension

VÍTEČEK, Jan, A. WUNSCHOVA, Jiří PETŘEK, Vojtěch ADAM, René KIZEK et. al.

Základní údaje

Originální název

Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY 2 cell suspension

Název česky

Buněčná smrt indukovaná nitroprusidem sodným a hydroghen peroxidem v buněčné suspenzi tobacco BY 2

Autoři

VÍTEČEK, Jan (203 Česká republika), A. WUNSCHOVA (203 Česká republika), Jiří PETŘEK (203 Česká republika), Vojtěch ADAM (203 Česká republika), René KIZEK (203 Česká republika, garant) a L. HAVEL (203 Česká republika)

Vydání

Biologica Plantarum, 2007, 0006-3134

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.259

Kód RIV

RIV/00216224:14310/07:00020265

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000247412400011

Klíčová slova anglicky

Cell death; sodium nitroprusside; hydrogen peroxide; tobacco BY 2 cell suspension

Štítky

cell death, hydrogen peroxide, sodium nitroprusside

Příznaky

Recenzováno

Změněno: 29. 5. 2007 10:48, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

The interplay between nitric oxide (NO) and reactive oxygen species can lead to an induction of cell death in plants. The aim of our work was to find out if cyanide released from sodium nitroprusside (SNP; a donor of NO) could be involved in the cell death induction, which is triggered by SNP and H2O2. Cell suspension of Nicotiana tabacum L. (line BY-2) was treated with 0.5 mM SNP, 0.5 mM potassium ferricyanide (PFC; analogue of sodium nitroprusside which can not release NO) and/or by 0.5 mM glucose with 0.5 U cm-3 glucose oxidase (GGO; a donor system of H2O2). The cell death was induced only by combination of SNP and GGO. Thus cyanide released was not involved in the induction of cell death. However, SNP showed toxic effect because of decrease in activities of intracellular oxidoreductases and esterases. The cell death caused by SNP and GGO occurred within 12 h. During cell death either length or width of the cell increased. Central vacuole was formed in 20 to 40 % of cells. Most of the dead cells showed a condensed cytoplasm. Two hallmarks of programmed cell death (PCD), chromatin condensation and blebbing of nuclear periphery, were observed. However, oligonucleosomal fragmentation of DNA, another hallmark of PCD, was not detected.

Česky

Buněčná smrt indukovaná nitroprusidem sodným a hydroghen peroxidem v buněčné suspenzi tobacco BY 2

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

Citovat

VÍTEČEK, Jan, A. WUNSCHOVA, Jiří PETŘEK, Vojtěch ADAM, René KIZEK a L. HAVEL. Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY 2 cell suspension. Biologica Plantarum. 2007, roč. 51, č. 3, s. 472-479. ISSN 0006-3134.

@article{718621,
   author = {Víteček, Jan and Wunschova, A. and Petřek, Jiří and Adam, Vojtěch and Kizek, René and Havel, L.},
   article_number = {3},
   keywords = {Cell death; sodium nitroprusside; hydrogen peroxide; tobacco BY 2 cell suspension},
   language = {eng},
   issn = {0006-3134},
   journal = {Biologica Plantarum},
   title = {Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY 2 cell suspension},
   volume = {51},
   year = {2007}
}

TY  - JOUR
ID  - 718621
AU  - Víteček, Jan - Wunschova, A. - Petřek, Jiří - Adam, Vojtěch - Kizek, René - Havel, L.
PY  - 2007
TI  - Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY 2 cell suspension
JF  - Biologica Plantarum
VL  - 51
IS  - 3
SP  - 472-479
EP  - 472-479
SN  - 00063134
KW  - Cell death
KW  - sodium nitroprusside
KW  - hydrogen peroxide
KW  - tobacco BY 2 cell suspension
N2  - The interplay between nitric oxide (NO) and reactive oxygen species can lead to an induction of cell death in plants. The aim of our work was to find out if cyanide released from sodium nitroprusside (SNP; a donor of NO) could be involved in the cell death induction, which is triggered by SNP and H2O2. Cell suspension of Nicotiana tabacum L. (line BY-2) was treated with 0.5 mM SNP, 0.5 mM potassium ferricyanide (PFC; analogue of sodium nitroprusside which can not release NO) and/or by 0.5 mM glucose with 0.5 U cm-3 glucose oxidase (GGO; a donor system of H2O2). The cell death was induced only by combination of SNP and GGO. Thus cyanide released was not involved in the induction of cell death. However, SNP showed toxic effect because of decrease in activities of intracellular oxidoreductases and esterases. The cell death caused by SNP and GGO occurred within 12 h. During cell death either length or width of the cell increased. Central vacuole was formed in 20 to 40 % of cells. Most of the dead cells showed a condensed cytoplasm. Two hallmarks of programmed cell death (PCD), chromatin condensation and blebbing of nuclear periphery, were observed. However, oligonucleosomal fragmentation of DNA, another hallmark of PCD, was not detected.
ER  -

VÍTEČEK, Jan, A. WUNSCHOVA, Jiří PETŘEK, Vojtěch ADAM, René KIZEK a L. HAVEL. Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY 2 cell suspension. \textit{Biologica Plantarum}. 2007, roč.~51, č.~3, s.~472-479. ISSN~0006-3134.

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