A new tool for distinguishing of different structural forms of
lactoferrin

J 2007

A new tool for distinguishing of different structural forms of lactoferrin

KUKAČKA, J.; Ondřej ZÍTKA; A. HORNA; Karel STEJSKAL; Josef ZEHNÁLEK et al.

Základní údaje

Originální název

A new tool for distinguishing of different structural forms of lactoferrin

Název česky

Nový nástroj pro rozdělení rozdílných strukturních forem laktoferinu

Autoři

KUKAČKA, J.; Ondřej ZÍTKA; A. HORNA; Karel STEJSKAL; Josef ZEHNÁLEK; Vojtěch ADAM; L. HAVEL; L. ZEMAN; R. PRŮŠA; Libuše TRNKOVÁ a René KIZEK

Vydání

Faseb J. 2007, 0892-6638

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.791

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/07:00022110

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000245708505187

Klíčová slova anglicky

distinguish; structural forms; lactoferrin; FIA-ED

Štítky

distinguish, FIA-ED, Lactoferrin, structural forms

Změněno: 29. 5. 2007 13:53, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

Lactoferrin is considered a multifunctional or multi-tasking protein. It appears to play several biological roles, whereas its structure is very crucial. The aim of this work was to investigate basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect to study of structural changes of the compound of interest. The behaviour of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection for determination of lactoferrin. We found out that the most suitable FIA-ED conditions were as follows: working electrode potential of 900 mV, Britton-Robinson buffer (pH 4.5) as mobile phase, its flow rate 1 ml.min-1. The detection limit was about tens of ng per ml. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to changes in its structure. As we have shown here, electrochemical analysis enables to distinguish a change of protein structure easily and rapidly.

Česky

Nový nástroj pro rozdělení rozdílných strukturních forem laktoferinu

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

KUKAČKA, J.; Ondřej ZÍTKA; A. HORNA; Karel STEJSKAL; Josef ZEHNÁLEK; Vojtěch ADAM; L. HAVEL; L. ZEMAN; R. PRŮŠA; Libuše TRNKOVÁ a René KIZEK. A new tool for distinguishing of different structural forms of lactoferrin. Faseb J. 2007, roč. 21, č. 5, s. A635-A635, 1 s. ISSN 0892-6638.

@article{718640,
   author = {Kukačka, J. and Zítka, Ondřej and Horna, A. and Stejskal, Karel and Zehnálek, Josef and Adam, Vojtěch and Havel, L. and Zeman, L. and Průša, R. and Trnková, Libuše and Kizek, René},
   article_number = {5},
   keywords = {distinguish; structural forms; lactoferrin; FIA-ED},
   language = {eng},
   issn = {0892-6638},
   journal = {Faseb J.},
   title = {A new tool for distinguishing of different structural forms of lactoferrin},
   volume = {21},
   year = {2007}
}

TY  - JOUR
ID  - 718640
AU  - Kukačka, J. - Zítka, Ondřej - Horna, A. - Stejskal, Karel - Zehnálek, Josef - Adam, Vojtěch - Havel, L. - Zeman, L. - Průša, R. - Trnková, Libuše - Kizek, René
PY  - 2007
TI  - A new tool for distinguishing of different structural forms of lactoferrin
JF  - Faseb J.
VL  - 21
IS  - 5
SP  - A635-A635
EP  - A635-A635
SN  - 08926638
KW  - distinguish
KW  - structural forms
KW  - lactoferrin
KW  - FIA-ED
N2  - Lactoferrin is considered a multifunctional or multi-tasking protein. It appears to play several biological roles, whereas its structure is very crucial. The aim of this work was to investigate basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect to study of structural changes of the compound of interest. The behaviour of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection for determination of lactoferrin. We found out that the most suitable FIA-ED conditions were as follows: working electrode potential of 900 mV, Britton-Robinson buffer (pH 4.5) as mobile phase, its flow rate 1 ml.min-1. The detection limit was about tens of ng per ml. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to changes in its structure. As we have shown here, electrochemical analysis enables to distinguish a change of protein structure easily and rapidly.
ER  -

KUKAČKA, J.; Ondřej ZÍTKA; A. HORNA; Karel STEJSKAL; Josef ZEHNÁLEK; Vojtěch ADAM; L. HAVEL; L. ZEMAN; R. PRŮŠA; Libuše TRNKOVÁ a René KIZEK. A new tool for distinguishing of different structural forms of lactoferrin. \textit{Faseb J.}. 2007, roč.~21, č.~5, s.~A635-A635, 1 s. ISSN~0892-6638.

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