Sequencing of Treponema pallidum ssp. pertenue Samoa D genome.

D 2007

Sequencing of Treponema pallidum ssp. pertenue Samoa D genome.

MATĚJKOVÁ, Petra, Michal STROUHAL, David ŠMAJS, S. J. NORRIS, G. M. WEINSTOCK et. al.

Basic information

Original name

Sequencing of Treponema pallidum ssp. pertenue Samoa D genome.

Name in Czech

Sekvencování genomu Treponema pallidum ssp. pertenue Samoa D.

Authors

MATĚJKOVÁ, Petra, Michal STROUHAL, David ŠMAJS, S. J. NORRIS and G. M. WEINSTOCK

Edition

Munich, Germany, In Abstract Book: 17th European Congress of Clinical Microbiology and Infectous Diseases. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. p. 238-238, 2007

Publisher

European Congress of Clinical Microbiology and Infectous Diseases

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.338

Organization unit

Faculty of Medicine

ISSN

UT WoS

000253692400750

Keywords in English

Sequencing; Treponema pallidum ssp. pertenue Samoa D

Abstract

ORIG CZ

V originále

Although syphilis and yaws have different clinical manifestation, the pathogens are microscopically and serologically indistinguishable. Genome scale approaches (microarray hybridisations and whole genome fingerprinting, WGF) showed that there are no gene deletions and multiplications and no large indels. Relatively subtle genetic differences responsible for different pathogenesis of syphilis and yaws thus require complete genome sequence of TPE. Physical map of complete genome sequence obtained by combination of CGS and DDT of heterologous regions was confirmed by WGF. CGS together with DDT sequencing and WGF was employed to obtain complete genome sequence of TPE Samoa D strain. The unfinished complete genome sequence comprised 1 linear contig. In silico proteome analysis revealed that more than 300 proteins (out of 1039 proteins predicted in TPA Nichols strain) were affected by sequence changes. The genome sequence of yaws treponeme was used to define intersubspecies difference between TPE and TPA.

In Czech

Genom Treponema pallidum ssp. pertenue (byl sekvencován dvěma nezávislými technikami komparativním genomovým sekvencováním a pyrosekvencováním. Obě techniky byly porovnány.

Links

GA310/07/0321, research and development project

Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

NR8967, research and development project

Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministry of Health of the CR

Citovat

MATĚJKOVÁ, Petra, Michal STROUHAL, David ŠMAJS, S. J. NORRIS and G. M. WEINSTOCK. Sequencing of Treponema pallidum ssp. pertenue Samoa D genome. In In Abstract Book: 17th European Congress of Clinical Microbiology and Infectous Diseases. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS. Munich, Germany: European Congress of Clinical Microbiology and Infectous Diseases, 2007, p. 238. ISSN 0924-8579.

@inproceedings{720038,
   author = {Matějková, Petra and Strouhal, Michal and Šmajs, David and Norris, S. J. and Weinstock, G. M.},
   address = {Munich, Germany},
   booktitle = {In Abstract Book: 17th European Congress of Clinical Microbiology and Infectous Diseases. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS.},
   keywords = {Sequencing; Treponema pallidum ssp. pertenue Samoa D},
   language = {eng},
   location = {Munich, Germany},
   pages = {238-238},
   publisher = {European Congress of Clinical Microbiology and Infectous Diseases},
   title = {Sequencing of Treponema pallidum ssp. pertenue Samoa D genome.},
   year = {2007}
}

TY  - JOUR
ID  - 720038
AU  - Matějková, Petra - Strouhal, Michal - Šmajs, David - Norris, S. J. - Weinstock, G. M.
PY  - 2007
TI  - Sequencing of Treponema pallidum ssp. pertenue Samoa D genome.
PB  - European Congress of Clinical Microbiology and Infectous Diseases
CY  - Munich, Germany
KW  - Sequencing
KW  - Treponema pallidum ssp. pertenue Samoa D
N2  - Although syphilis and yaws have different clinical manifestation, the pathogens are microscopically and serologically indistinguishable. Genome scale approaches (microarray hybridisations and whole genome fingerprinting, WGF) showed that there are no gene deletions and multiplications and no large indels. Relatively subtle genetic differences responsible for different pathogenesis of syphilis and yaws thus require complete genome sequence of TPE. Physical map of complete genome sequence obtained by combination of CGS and DDT of heterologous regions was confirmed by WGF. CGS together with DDT sequencing and WGF was employed to obtain complete genome sequence of TPE Samoa D strain. The unfinished complete genome sequence comprised 1 linear contig. In silico proteome analysis revealed that more than 300 proteins (out of 1039 proteins predicted in TPA Nichols strain) were affected by sequence changes. The genome sequence of yaws treponeme was used to define intersubspecies difference between TPE and TPA.
ER  -

MATĚJKOVÁ, Petra, Michal STROUHAL, David ŠMAJS, S. J. NORRIS and G. M. WEINSTOCK. Sequencing of Treponema pallidum ssp. pertenue Samoa D genome. In \textit{In Abstract Book: 17th European Congress of Clinical Microbiology and Infectous Diseases. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS.}. Munich, Germany: European Congress of Clinical Microbiology and Infectous Diseases, 2007, p.~238. ISSN~0924-8579.

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