2007
Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection
KLEPÁRNÍK, Martin a Josef TOMANDLZákladní údaje
Originální název
Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection
Název česky
Stanovení asymetrického dimethylargininu v lidské plazmě pomocí HPLC s coulometrickou detekcí
Autoři
KLEPÁRNÍK, Martin a Josef TOMANDL
Vydání
Belgium, HPLC 2007. Abstract Book. od s. 909-909, 1 s. 2007
Nakladatel
I.O.P.M.S. vzw
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Belgie
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
asymmetric dimethylarginine; HPLC; coularray detection
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 26. 9. 2007 17:32, doc. RNDr. Josef Tomandl, Ph.D.
V originále
Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection.
Česky
Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection.
Návaznosti
| GA203/06/0047, projekt VaV |
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| LC06023, projekt VaV |
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| MSM0021622402, záměr |
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