2007
AhR-mediated and antiestrogenic activity of humic substances
JANOŠEK, Jaroslav, Michal BITTNER, Klára HILSCHEROVÁ, Luděk BLÁHA, J.P. GIESY et. al.Základní údaje
Originální název
AhR-mediated and antiestrogenic activity of humic substances
Název česky
AhR-zprostředkovaná a antiestrogenní aktivita huminových látek
Autoři
JANOŠEK, Jaroslav (203 Česká republika), Michal BITTNER (203 Česká republika), Klára HILSCHEROVÁ (203 Česká republika, garant), Luděk BLÁHA (203 Česká republika), J.P. GIESY (840 Spojené státy) a Ivan HOLOUBEK (203 Česká republika)
Vydání
Chemosphere, Oxford, UK, Elsevier Science Ltd. 2007, 0045-6535
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30304 Public and environmental health
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.739
Kód RIV
RIV/00216224:14310/07:00020344
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000245846700005
Klíčová slova anglicky
Ah receptor;AhR-mediated activity;Antiestrogenic effects;Endocrine disruption
Změněno: 18. 3. 2010 10:16, prof. RNDr. Luděk Bláha, Ph.D.
V originále
Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10-8-7.4 x 10-8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists. Contribution of persistent organic compounds to the observed effects was ruled out by H2SO4 treatment. Eight out of twelve HS elicited significant antiestrogenic effects with IC50 ranging from 40 to 164 mg l-1. The possible explanations of the antiestrogenic effect include sorption of 17-[beta]-estradiol (E2) on HS, changes in membrane permeability for E2 or another specific mechanism.
Česky
doplnit
Návaznosti
| GP525/05/P160, projekt VaV |
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| MSM0021622412, záměr |
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