2007
Changes of AhR-mediated activity of humic substances after irradiation
BITTNER, Michal, Klára HILSCHEROVÁ a John, Paul GIESYZákladní údaje
Originální název
Changes of AhR-mediated activity of humic substances after irradiation
Název česky
Změny AhR-zprostředkované aktivity huminových látek po ozáření
Autoři
BITTNER, Michal (203 Česká republika, garant), Klára HILSCHEROVÁ (203 Česká republika) a John, Paul GIESY (840 Spojené státy)
Vydání
Environment International, Amsterdam, The Netherlands, Elsevier, 2007, 0160-4120
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30304 Public and environmental health
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.797
Kód RIV
RIV/00216224:14310/07:00020354
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000248273700013
Klíčová slova anglicky
Humic acids; Ah receptor; H4IIE-luc; Absorbance; NOM
Štítky
Změněno: 18. 3. 2010 10:45, prof. RNDr. Luděk Bláha, Ph.D.
V originále
Humic substances (HS) and natural organic matter (NOM) are natural organic compounds ubiquitous in the environment. However, some studies indicate that both HS and NOM can act as xenobiotics, e.g. induce hormone like effects in fish, amphibians and invertebrates. Molecules of these substances contain a number of aromatic rings and conjugated double bonds, the so called chromophores. Irradiation of dissolved HS and NOM can lead to a series of photochemical reactions which can act on these substances itself, or on other substances present in aquatic environment along with HS and NOM such as e.g. xenobiotics. In our previous study, we have found significant interactions of five humic acids (HA) with cytosolic aryl hydrocarbon receptor (AhR) in an in vitro bioassay based on H4IIEluc cells. In the present study, we have studied the changes in AhR mediated activities both of HS and NOM after irradiation that simulated natural solar light. Nine different HS and two NOM samples were irradiated in Pyrex tubes with a medium pressure mercury lamp for a duration of 0 to 52 h (which corresponds to 0 to 52 d natural solar radiation). Original concentrations of the samples were 50 mg L, and the greatest concentration of HS and NOM photoproducts subsequently tested in the bioassay was 17 mg L, which is an environmentally relevant concentration. After irradiation the absorbances of all the samples were less than the original materials. The AhR mediated activity of the HA Fluka and HA Sodium Salt were partially decreased by irradiation. The activities of other HS and NOM, that were either AhR active or inactive were not changed by irradiation. The results of the study demonstrate that AhRmediated activities of two active HA is caused by both photostable and photolabile AhR activators, while the other three active HA contain only photostable AhR activators. Potential mechanisms of the observed irradiation induced changes in AhRmediated activities are discussed.
Česky
Viz Anglický, výsledky experimentů Změny AhR-zprostředkované aktivity huminových látek po ozáření
Návaznosti
| GP525/05/P160, projekt VaV |
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| MSM0021622412, záměr |
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