SUCHOMELOVÁ, Jana, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ, Hana PAULOVÁ and Pavel MUSIL. HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae. Online. Journal of Pharmaceutical and Biomedical Analysis. 2007, vol. 44, No 1, p. 283-287. ISSN 0731-7085. [citováno 2024-04-23]
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Basic information
Original name HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae
Name in Czech HPLC analýza sedmi kvartérních benzofenanthridinových alkaloidů obsažených v šesti druzích čeledi Papaveraceae
Authors SUCHOMELOVÁ, Jana (203 Czech Republic), Eva TÁBORSKÁ (203 Czech Republic, guarantor), Hana BOCHOŘÁKOVÁ (203 Czech Republic), Hana PAULOVÁ (203 Czech Republic) and Pavel MUSIL (203 Czech Republic)
Edition Journal of Pharmaceutical and Biomedical Analysis, 2007, 0731-7085.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 40100 4.1 Agriculture, Forestry, and Fisheries
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.761
RIV identification code RIV/00216224:14110/07:00020380
Organization unit Faculty of Medicine
UT WoS 000246350100041
Keywords in English Benzo[c]phenanthridine; Chelilutine; Chelirubine; Macarpine; Sanguirubine; Sanguilutine; Sanguinarine; Chelerythrine; RP-HPLC
Tags benzo[c]phenanthridine, Chelerythrine, Chelilutine, Chelirubine, Macarpine, RP-HPLC, Sanguilutine, sanguinarine, Sanguirubine
Tags Reviewed
Changed by Changed by: RNDr. Hana Paulová, CSc., učo 1355. Changed: 23/6/2009 08:18.
Abstract
The content of the seven quaternary benzophenanthridine alkaloids (QBA) sanguinarine (SA), chelerythrine (CHE), chelirubine (CHR), chelilutine (CHL), sanguilutine (SL), sanguirubine (SR) and macarpine (MA) was determined in the underground part of six plant species of the family Papaveraceae (Sanguinaria canadensis L., Dicranostigma lactucoides HOOK.f.et THOMS, Chelidonium majus L., Macleaya cordata (Willd.), Macleaya microcarpa (Maxim) and Stylophorum lasiocarpum (Oliv.)). HPLC method with reversed phase column Synergi Max RP C12 Phenomenex was used, mobile phase consisted of heptanesulfonic acid (0.01 mol/l) with triethanolamine (0.1 mol/l) in redistilled water, pH 2.5, acetonitrile gradient 25 60% during 25 min. Detection was performed at 280 nm. The highest content of SA and CHE was found in the roots of D. lactucoides (1.99%, resp. 3.43% of the dried roots). In rhizomes of S. canadensis was their content more then two times lower.
Abstract (in Czech)
V podzemních částech šesti druhů čeledi Papaveraceae (Sanguinaria canadensis L., Dicranostigma lactucoides HOOK.f.et THOMS, Chelidonium majus L., Macleaya cordata (Willd.), Macleaya microcarpa (Maxim) a Stylophorum lasiocarpum (Oliv.)) byl stanoven obsah jednotlivých kvartérních benzofenanthridinových alkaloidů : sanguinarinu (SA), chelerythrinu (CHE), chelirubinu (CHR), chelilutinu (CHL), sanguilutinu (SL), sanguirubinu (SR) a macarpinu (MA). Byla použita RP-HPLC metoda, kde byla použita jako stacionární fáze kolona Synergi Max RP C 12 Phenomenex, mobilní fáze byla složena z heptansulfonové kyseliny (0,01mol/l) s triethanolaminem (0,1 mol/l) v redestilované vodě s pH 2,5 a acetonitrilovým gradientem 25 60% během 25 minut. Detekce byla prováděna při vlnové délce 280 nm. Nejvyšší obsah SA a CHE byl nalezen v kořenech D. lactucoides (1,99%, resp. 3,43% suchého kořene). V kořenech S. canadensis byl jejich obsah přibližně dvakrát menší.
Links
GA525/04/0017, research and development projectName: Rostlinné zdroje benzofenanthridinových alkaloidů a účinek těchto alkaloidů na normální a nádorové buňky
Investor: Czech Science Foundation, Plant sources of benzophenanthridine alkaloids and effects of these alkaloid on normal and cancer cells
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