PALEČEK, Emil, Michal MASAŘÍK, René KIZEK, Dirk KUHLMEIER, Jörg HASSMANN and Jürgen SCHÜLEIN. Sensitive electrochemical determination of unlabeled MutS protein and detection of point mutations in DNA. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2004, vol. 76, No 19, p. 5930-5936. ISSN 0003-2700. |
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@article{721757, author = {Paleček, Emil and Masařík, Michal and Kizek, René and Kuhlmeier, Dirk and Hassmann, Jörg and Schülein, Jürgen}, article_location = {Washington, D.C., USA}, article_number = {19}, keywords = {MEDIATED CHARGE-TRANSPORT; MODIFIED ELECTRODES; NUCLEIC-ACIDS; STRIPPING VOLTAMMETRY; BIOACTIVE PEPTIDES; MERCURY-ELECTRODES; CARBON ELECTRODES; MISMATCH REPAIR; SOLID AMALGAMS; HYBRIDIZATION}, language = {eng}, issn = {0003-2700}, journal = {Analytical Chemistry}, title = {Sensitive electrochemical determination of unlabeled MutS protein and detection of point mutations in DNA}, volume = {76}, year = {2004} }
TY - JOUR ID - 721757 AU - Paleček, Emil - Masařík, Michal - Kizek, René - Kuhlmeier, Dirk - Hassmann, Jörg - Schülein, Jürgen PY - 2004 TI - Sensitive electrochemical determination of unlabeled MutS protein and detection of point mutations in DNA JF - Analytical Chemistry VL - 76 IS - 19 SP - 5930-5936 EP - 5930-5936 PB - American Chemical Society SN - 00032700 KW - MEDIATED CHARGE-TRANSPORT KW - MODIFIED ELECTRODES KW - NUCLEIC-ACIDS KW - STRIPPING VOLTAMMETRY KW - BIOACTIVE PEPTIDES KW - MERCURY-ELECTRODES KW - CARBON ELECTRODES KW - MISMATCH REPAIR KW - SOLID AMALGAMS KW - HYBRIDIZATION N2 - MutS protein plays an important role in the DNA repair system in prokaryotic and eukaryotic cells; it recognizes unpaired and mispaired bases in duplex DNA and can be used for detection of point mutations in vitro. We have shown that small amounts of this protein can be detected electrochemically at mercury and carbon electrodes without any labeling. Using constant current stripping analysis (CPSA) and mercury electrodes, tens of attomoles of this protein can be detected. The sensitivity of the determination at carbon electrodes is by more than 3 orders of magnitude lower. Using biotinylated DNA duplexes attached to magnetic beads, single-base mismatches and insertion/deletions were recognized by MutS. Picogram amounts of this protein were detected by CPSA after MutS releasing from the beads. ER -
PALEČEK, Emil, Michal MASAŘÍK, René KIZEK, Dirk KUHLMEIER, Jörg HASSMANN and Jürgen SCHÜLEIN. Sensitive electrochemical determination of unlabeled MutS protein and detection of point mutations in DNA. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2004, vol.~76, No~19, p.~5930-5936. ISSN~0003-2700.
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