Changes of nephron of laboratory rat after administration of
Amphotericin B colloidal dispersion and conventional
Amphotericin B deoxycholate -morphology and morphometry study

D 2004

Changes of nephron of laboratory rat after administration of Amphotericin B colloidal dispersion and conventional Amphotericin B deoxycholate -morphology and morphometry study

KREJČÍŘOVÁ, Lenka, Irena LAUSCHOVÁ, Drahomír HORKÝ, Michael DOUBEK, Jiří MAYER et. al.

Základní údaje

Originální název

Changes of nephron of laboratory rat after administration of Amphotericin B colloidal dispersion and conventional Amphotericin B deoxycholate -morphology and morphometry study

Název česky

Změny nefronu laboratorního potkana po podání Amphotericinu B a konvenčního Amphotericinu B deoxycholátu - morfologická a morfometrická studie

Autoři

KREJČÍŘOVÁ, Lenka, Irena LAUSCHOVÁ, Drahomír HORKÝ, Michael DOUBEK, Jiří MAYER a Jaromír DOUBEK

Vydání

Košice, Sborník abstrakt 42.kongresu Slovenské anatomické společnosti s mezinárodní účastí, od s. 48-48, 1 s. 2004

Nakladatel

Edičné stredisko UPJŠ

Další údaje

Typ výsledku

Stať ve sborníku

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Změněno: 14. 9. 2007 14:14, MUDr. Lenka Krejčířová, Ph.D.

Anotace

ORIG CZ

V originále

In the light microscopy changes of structure of proximal and distal tubules of cortex with implication of necrosis were observed. An extensive damage of brush border area was mainly observed in the case of proximal tubules. We did not observe severe histopathological changes in the medulla especially after administration of ABCD. In the electron microscopy in the cortex the enlargement of extracellular spaces of basal labyrinth, especially expressive in the distal tubules were observed. Cell apices protruded in lumina in numerous cytoplasmic buds lacking cell organelles. It was observed in the cytoplasm of cells in both tubules to their basic healing lysosomes, pathological changes of mitochondria and many vacuoles. In the medulla of kidney were in area of basic of proximal tubules dilatation of extracellular spaces and in the cells of tubuli were numerous lysosomes and big pathological changes in mitochondria. Changes of tubules described in the cortex and the medulla were frequently seen after administration of AmB. In good accordance with qualitative findings were results of morphometric observations. Percentage of damaged mitochondria was established – values in both tubules were approximately twice higher in group of animals, to which AmB deoxycholate was applied. Statisticaly significant diference was observed in density of vacuoles and peroxisomes. AmB deoxycholate causes considerably more severe damage to tubules but the morphological damage to renal glomeruli is minimal in both formulas. In tubular cells, AmB deoxycholate causes more than ABCD damage to mitochondria, vacuolation of cytoplasm, and increased values of volume density of peroxisomes.

Česky

Cílem studie bylo srovnání morfologických změn ledvinné tkáně laboratorního potkana po podání AmB a ABCD. Předběžné klinické studie totiž nevykazovaly výrazné rozdíly mezi oběma léky a dle funkčních vyšetření se zdálo, že jsou si obě látky rovnocenné.Na základě našich pozorování usuzujeme, že obě látky poškozují po delší aplikaci zřejmě nekroticky ledvinnou tkáň, ale ABCD vykazuje morfologické změny nižší intenzity.

Návaznosti

NL6514, projekt VaV

Název: Jednoduché nefroprotektivní postupy v profylaxi nefrotoxicity konvenčního amfotericinu B u onkologických pacientů

Investor: Ministerstvo zdravotnictví ČR, Jednoduché nefroprotektivní postupy v profylaxi nefrotoxicity konvenčního amfotericinu B u onkologických pacientů

Citovat

KREJČÍŘOVÁ, Lenka, Irena LAUSCHOVÁ, Drahomír HORKÝ, Michael DOUBEK, Jiří MAYER a Jaromír DOUBEK. Changes of nephron of laboratory rat after administration of Amphotericin B colloidal dispersion and conventional Amphotericin B deoxycholate -morphology and morphometry study. In Sborník abstrakt 42.kongresu Slovenské anatomické společnosti s mezinárodní účastí. Košice: Edičné stredisko UPJŠ, 2004, s. 48-48.

@inproceedings{724985,
   author = {Krejčířová, Lenka and Lauschová, Irena and Horký, Drahomír and Doubek, Michael and Mayer, Jiří and Doubek, Jaromír},
   address = {Košice},
   booktitle = {Sborník abstrakt 42.kongresu Slovenské anatomické společnosti s mezinárodní účastí},
   location = {Košice},
   pages = {48-48},
   publisher = {Edičné stredisko UPJŠ},
   title = {Changes of nephron of laboratory rat after administration of Amphotericin B colloidal dispersion and conventional Amphotericin B deoxycholate -morphology and morphometry study},
   year = {2004}
}

TY  - CONF
ID  - 724985
AU  - Krejčířová, Lenka - Lauschová, Irena - Horký, Drahomír - Doubek, Michael - Mayer, Jiří - Doubek, Jaromír
PY  - 2004
TI  - Changes of nephron of laboratory rat after administration of Amphotericin B colloidal dispersion and conventional Amphotericin B deoxycholate -morphology and morphometry study
PB  - Edičné stredisko UPJŠ
CY  - Košice
N2  - In the light microscopy changes of structure of proximal and distal tubules of cortex with implication of necrosis were observed. An extensive damage of brush border area was mainly observed in the case of proximal tubules. We did not observe severe histopathological changes in the medulla especially after administration of ABCD. In the electron microscopy in the cortex the enlargement of extracellular spaces of basal labyrinth, especially expressive in the distal tubules were observed. Cell apices protruded in lumina in numerous cytoplasmic buds lacking cell organelles. It was observed in the cytoplasm of cells in both tubules to their basic healing lysosomes, pathological changes of mitochondria and many vacuoles. In the medulla of kidney were in area of basic of proximal tubules dilatation of extracellular spaces and in the cells of tubuli were numerous lysosomes and big pathological changes in mitochondria. Changes of tubules described in the cortex and the medulla were frequently seen after administration of AmB. In good accordance with qualitative findings were results of morphometric observations. Percentage of damaged mitochondria was established – values in both tubules were approximately twice higher in group of animals, to which AmB deoxycholate was applied. Statisticaly significant diference was observed in density of vacuoles and peroxisomes. AmB deoxycholate causes considerably more severe damage to tubules but the morphological damage to renal glomeruli is minimal in both formulas. In tubular cells, AmB deoxycholate causes more than ABCD damage to mitochondria, vacuolation of cytoplasm, and increased values of volume density of peroxisomes.
ER  -

KREJČÍŘOVÁ, Lenka, Irena LAUSCHOVÁ, Drahomír HORKÝ, Michael DOUBEK, Jiří MAYER a Jaromír DOUBEK. Changes of nephron of laboratory rat after administration of Amphotericin B colloidal dispersion and conventional Amphotericin B deoxycholate -morphology and morphometry study. In \textit{Sborník abstrakt 42.kongresu Slovenské anatomické společnosti s mezinárodní účastí}. Košice: Edičné stredisko UPJŠ, 2004, s.~48-48.

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