| KAŠPÁREK, Petr, Roman PANTŮČEK, Jana KAHÁNKOVÁ, Vladislava RŮŽIČKOVÁ a Jiří DOŠKAŘ. Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812. Folia Microbiologica. Praha: Czechoslovak Society for Microbiology, 2007, roč. 52, č. 4, s. 331-338. ISSN 0015-5632. |
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@article{725473,
author = {Kašpárek, Petr and Pantůček, Roman and Kahánková, Jana and Růžičková, Vladislava and Doškař, Jiří},
article_location = {Praha},
article_number = {4},
keywords = {Staphylococcus bacteriophage; host-range; bacteriophage therapy; introns},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812},
url = {http://www.cssm.info/priloha/fm2007_331.pdf},
volume = {52},
year = {2007}
}
TY - JOUR
ID - 725473
AU - Kašpárek, Petr - Pantůček, Roman - Kahánková, Jana - Růžičková, Vladislava - Doškař, Jiří
PY - 2007
TI - Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812
JF - Folia Microbiologica
VL - 52
IS - 4
SP - 331-338
EP - 331-338
PB - Czechoslovak Society for Microbiology
SN - 00155632
KW - Staphylococcus bacteriophage
KW - host-range
KW - bacteriophage therapy
KW - introns
UR - http://www.cssm.info/priloha/fm2007_331.pdf
N2 - Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and coagulase-negative Staphylococcus species. Mutational changes in the genome of several previously isolated host-range mutants of phage 812 were identified and described. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Mutants 812i, 812b, 812p and 812F3 harbor deletion in the structural gene orf8 that results from a genome rearrangement that is associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages, U16 and phi131. Another intron was discovered in the recA812 gene in mutants 812i, 812b, 812p and 812F3. An insertion was found in a non-coding region of the restriction fragment PstI-O of mutants 812b, 812F3 and 812g and phages U16 and phi131. Results of this study contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages.
ER -
KAŠPÁREK, Petr, Roman PANTŮČEK, Jana KAHÁNKOVÁ, Vladislava RŮŽIČKOVÁ a Jiří DOŠKAŘ. Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812. \textit{Folia Microbiologica}. Praha: Czechoslovak Society for Microbiology, 2007, roč.~52, č.~4, s.~331-338. ISSN~0015-5632.
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