An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ a Radim JANČÁLEK. An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve. In European Glial Cell Meeting. 2007. ISBN 1740-925X.

Základní údaje

• Originální název: An immunohistochemical localization of SDF-1 and its receptor CXCR4 in dorsal root ganglia associated and non-associated to damaged nerve
• Název česky: Imunohistochemická lokalizace SDF-1 a jeho receptoru CXCR4 ve spinálních gangliích
• Autoři: DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ a Radim JANČÁLEK.
• Vydání: European Glial Cell Meeting, 2007.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Prezentace na konferencích
• Obor: 30000 3. Medical and Health Sciences
• Stát vydavatele: Velká Británie a Severní Irsko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Organizační jednotka: Lékařská fakulta
• ISBN: 1740-925X
• Klíčová slova anglicky: satellite cells; chemokine; chemokine receptor
• Štítky: chemokine, chemokine receptor, satellite cells
• Příznaky: Mezinárodní význam

Anotace

The biological activity of stromal cell-derived factor (SDF1) is signaled through the chemokine receptor CXCR4. An immunohistochemical location of SDF-1 and CXCR4 proteins was investigated in the C7-C8 and L4-L5 dorsal root ganglia (DRG) of naive rats and those operated for unilateral L4-L5 spinal nerve ligature (SNL) and sciatic nerve ligature (ScNL). The naive DRG displayed immunofluorescence for SDF1-IF and CXCR4-IF at the surface of neurons and satellite glial cells (SGC). A significant decrease of SDF1-IF was induced in the ipsilateral, but remained distinct in the contralateral L4-L5 DRG one week after SNL. A bilateral reduction of SDF1-IF was found 2 weeks from SNL. A gradual diminution of SDF1-IF was observed bilaterally in the L4-L5 DRG neurons, but modest IF was in SGC after 1 and 2 weeks from ScNL, respectively. The SNL for 1 week induced a reduction of CXCR4-IF at the SGC surfaces of ipsi- in contrast to contralateral L4-L5 DRG, while intraneuronal staining was increased. Two weeks after SNL, the differences of CXCR4-IF at the neuronal surfaces in ipsilateral L4-L5 DRG and at the SGC surfaces in contralateral ones were marked. CXCR4-IF at the SGC surfaces remained in both ipsi- and contralateral DRG up to 2 weeks from ScNL, but it was increased intraneuronally only in the ipsilateral DRG. Similar pattern of SDF1- and CXCR4-IF was induced in the cervical DRG. A different expression of SDF1-IF and its receptor was observed in ipsi- and contralateral DRG associated and non-associated with unilateral SNL and ScNL. This suggests their different regulation with the time of survival and type of nerve injury.

Anotace česky

Biologická aktivita SDF1 (stromal cell-derived factor) je zprostředkovaná chemokinovým receptorem CXCR4. Imunohistochemická lokaůizace SDF-1 a CXCR4 proteinů byla sledována v C7-C8 a L4-L5 spinálních gangliích intaktních a operovaných potkanů pro unilaterální ligaturu L4-L5 spinálního nervu SNL) a sedacího nervu (ScNL). The naive DRG displayed immunofluorescence for SDF1-IF and CXCR4-IF at the surface of neurons and satellite glial cells (SGC). A significant decrease of SDF1-IF was induced in the ipsilateral, but remained distinct in the contralateral L4-L5 DRG one week after SNL. A bilateral reduction of SDF1-IF was found 2 weeks from SNL. A gradual diminution of SDF1-IF was observed bilaterally in the L4-L5 DRG neurons, but modest IF was in SGC after 1 and 2 weeks from ScNL, respectively. The SNL for 1 week induced a reduction of CXCR4-IF at the SGC surfaces of ipsi- in contrast to contralateral L4-L5 DRG, while intraneuronal staining was increased. Two weeks after SNL, the differences of CXCR4-IF at the neuronal surfaces in ipsilateral L4-L5 DRG and at the SGC surfaces in contralateral ones were marked. CXCR4-IF at the SGC surfaces remained in both ipsi- and contralateral DRG up to 2 weeks from ScNL, but it was increased intraneuronally only in the ipsilateral DRG. Similar pattern of SDF1- and CXCR4-IF was induced in the cervical DRG. A different expression of SDF1-IF and its receptor was observed in ipsi- and contralateral DRG associated and non-associated with unilateral SNL and ScNL. This suggests their different regulation with the time of survival and type of nerve injury.

Návaznosti

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