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@inproceedings{725764,
author = {Španová, Alena and Kšicová, Kateřina and Rittich, Bohuslav and Krulová, Blanka and Doušková, Dagmar and Dráb, Vladimír and Drbohlav, Jan},
address = {Aas, Norway},
booktitle = {2nd Symposium on Propionibacteria and Bifidobacteria},
keywords = {Bifidobacterium; identification; PCR; ARDRA; rep-PCR; functional properties},
language = {eng},
location = {Aas, Norway},
pages = {12P},
publisher = {Norwegian University of Life Sciences, Aas, Norway},
title = {Genetic heterogeneity and functional properties of newly isolated probiotic Bifidobacterium species},
year = {2007}
}
TY - JOUR
ID - 725764
AU - Španová, Alena - Kšicová, Kateřina - Rittich, Bohuslav - Krulová, Blanka - Doušková, Dagmar - Dráb, Vladimír - Drbohlav, Jan
PY - 2007
TI - Genetic heterogeneity and functional properties of newly isolated probiotic Bifidobacterium species
PB - Norwegian University of Life Sciences, Aas, Norway
CY - Aas, Norway
KW - Bifidobacterium
KW - identification
KW - PCR
KW - ARDRA
KW - rep-PCR
KW - functional properties
N2 - Several molecular biological methods (genus-specific PCRs, species-specific PCRs, amplified ribosomal DNA restriction analysis (ARDRA), and rep-PCRs) were applied for the identification and discrimination of Bifidobacterium strains. The above-mentioned methods were used for identification and characterisation of 64 Bifidobacterium strains isolated from suckling babies and 12 Bifidobacterium strains isolated from healthy adults and were proved to be discriminatory methods for the species of bifidobacteria. All genotyping methods revealed small intraspecies heterogeneity. The most commonly detected probiotic species were B. longum, B. bifidum, B. adolescentis, and B. breve. In addition, B. animalis was identified. Some functional properties of selected bifidobacterial strains (surviving at low pH, resistance to bile salts, stability during storage, and influence on the growth of selected starter cultures used in milk industry) were proved. The properties of the bifidobacteria tested were strain-dependent.
ER -
ŠPANOVÁ, Alena, Kateřina KŠICOVÁ, Bohuslav RITTICH, Blanka KRULOVÁ, Dagmar DOUŠKOVÁ, Vladimír DRÁB and Jan DRBOHLAV. Genetic heterogeneity and functional properties of newly isolated probiotic Bifidobacterium species. In \textit{2nd Symposium on Propionibacteria and Bifidobacteria}. Aas, Norway: Norwegian University of Life Sciences, Aas, Norway, 2007, p.~12P, 1 pp.
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