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@inproceedings{726347,
author = {Onofrejová, Lucia and Farková, Marta and Preisler, Jan},
address = {Pardubice},
booktitle = {7th International Conference Vitamins, Nutrition and Diagnostics},
edition = {1. vyd.},
keywords = {quantification; artificial neural networks (ANN); matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS); fructooligosaccharides; raftilose; oligomer dispersion},
language = {eng},
location = {Pardubice},
isbn = {978-80-7194-937-4},
pages = {18-20},
publisher = {Univerzita Pardubice},
title = {Quantitative MALDI MS analysis of food oligosaccharides using artificial neural networks},
year = {2007}
}
TY - JOUR
ID - 726347
AU - Onofrejová, Lucia - Farková, Marta - Preisler, Jan
PY - 2007
TI - Quantitative MALDI MS analysis of food oligosaccharides using artificial neural networks
PB - Univerzita Pardubice
CY - Pardubice
SN - 9788071949374
KW - quantification
KW - artificial neural networks (ANN)
KW - matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS)
KW - fructooligosaccharides
KW - raftilose
KW - oligomer dispersion
N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) allows rapid determination of the oligosaccharides masses, oligomer dispersion and also has a potential for the quantitative analysis. In this study, method for MS quantification of food oligosaccharides using a standard addition of a pure monodisperse tetraoligosaccharide nystose with minimal optimisation including sampling and laser energy, was developed. Relative quantification was based on evaluation of change in peak intensity of four raftilose oligomers with degree of polymeration from 3 to 6 and assumption of constant oligomer dispersion profile. Raftilose content was evaluated with two mathematical approaches, conventional calculation and using artificial neural networks (ANN).
ER -
ONOFREJOVÁ, Lucia, Marta FARKOVÁ a Jan PREISLER. Quantitative MALDI MS analysis of food oligosaccharides using artificial neural networks. In \textit{7th International Conference Vitamins, Nutrition and Diagnostics}. 1. vyd. Pardubice: Univerzita Pardubice, 2007, s.~18-20. ISBN~978-80-7194-937-4.
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