A 2007

Molecular understanding of DSB repair-Mediator proteins

KREJČÍ, Lumír

Basic information

Original name

Molecular understanding of DSB repair-Mediator proteins

Name in Czech

Molekulární podstata opravy dvouřetězcových zlomů- Mediátorové proteiny

Authors

KREJČÍ, Lumír (203 Czech Republic, guarantor)

Edition

Heidelberg, 2007

Publisher

EMBO/HHMI

Other information

Language

English

Type of outcome

Audiovizuální tvorba

Field of Study

10600 1.6 Biological sciences

Country of publisher

Estonia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14310/07:00022864

Organization unit

Faculty of Science

Keywords in English

recombination; repair; DNA; cancer; Rad52; Srs2; mediator activity; SUMOylation

Tags

Tags

International impact
Změněno: 31/3/2010 11:33, doc. Mgr. Lumír Krejčí, Ph.D.

Abstract

ORIG CZ

V originále

DNA strand break repair by HR is mediated by genes of the RAD52 epistasis group. Rad52 protein plays central role in this process. It binds and anneals complementary ssDNA, self-associate to form multimeric rings, interacts with Rad51, and promotes nucleation of Rad51 onto ssDNA. Here, we show that Rad52 competes with Srs2 anti-recombinase in mediating Rad51-catalyzed strand exchange reaction through common interaction site on Rad51. The molecular mechanism of Rad52 function in homologous recombination together with the role of postranslational modification (SUMO-ylation) is discussed.

In Czech

V rámci této práce jsou prezentovány naše nové poznatky o proteinu Rad52 jeho kompetici s anti-rekombinčním proteinem Srs a regulaci pomocí kovaletní modifikace Smt3 peptidem.

Links

LC06030, research and development project
Name: Biomolekulární centrum
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre
ME 888, research and development project
Name: Srs2 protein a jeho multifunkční úloha při rekombinančních /opravných procesech
Investor: Ministry of Education, Youth and Sports of the CR, Srs2 protein and its multi-functional role in rekombination/repair processes
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment