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@article{727506,
author = {Pohanka, Miroslav and Skládal, Petr},
article_location = {Weinheim},
article_number = {23},
keywords = {Amperometric biosensor; Screen-printed sensor; Immunoassay; Francisella tularensis},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
note = {DOI 10.1002/elan.200704003},
title = {Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor},
url = {http://www3.interscience.wiley.com/cgi-bin/abstract/116320027/ABSTRACT?CRETRY=1&SRETRY=0},
volume = {19},
year = {2007}
}
TY - JOUR
ID - 727506
AU - Pohanka, Miroslav - Skládal, Petr
PY - 2007
TI - Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor
JF - Electroanalysis
VL - 19
IS - 23
SP - 2507-2512
EP - 2507-2512
PB - Wiley-VCH Verlag GmbH
SN - 10400397
N1 - DOI 10.1002/elan.200704003
KW - Amperometric biosensor
KW - Screen-printed sensor
KW - Immunoassay
KW - Francisella tularensis
UR - http://www3.interscience.wiley.com/cgi-bin/abstract/116320027/ABSTRACT?CRETRY=1&SRETRY=0
N2 - Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.
ER -
POHANKA, Miroslav a Petr SKLÁDAL. Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2007, roč.~19, č.~23, s.~2507-2512. ISSN~1040-0397.
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