Serological Diagnosis of Tularemia in Mice Using the
Amperometric Immunosensor

J 2007

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

POHANKA, Miroslav and Petr SKLÁDAL

Basic information

Original name

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

Name in Czech

Serologické stanovení tularemie u myší použitím amperometrického imunosensoru

Authors

POHANKA, Miroslav (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2007, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.949

RIV identification code

RIV/00216224:14310/07:00022879

Organization unit

Faculty of Science

UT WoS

000251891400003

Keywords in English

Amperometric biosensor; Screen-printed sensor; Immunoassay; Francisella tularensis

Abstract

ORIG CZ

V originále

Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.

In Czech

Bylo detekováno množství anti tularemických protilátek v séru myší infikovaných bakterií Francisella tularensis, pro stanovení byl využit amperometrický imunosensor na bázi sítotiskové elektrody.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

POHANKA, Miroslav and Petr SKLÁDAL. Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2007, vol. 19, No 23, p. 2507-2512. ISSN 1040-0397.

@article{727506,
   author = {Pohanka, Miroslav and Skládal, Petr},
   article_location = {Weinheim},
   article_number = {23},
   keywords = {Amperometric biosensor; Screen-printed sensor; Immunoassay; Francisella tularensis},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   note = {DOI 10.1002/elan.200704003},
   title = {Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor},
   url = {http://www3.interscience.wiley.com/cgi-bin/abstract/116320027/ABSTRACT?CRETRY=1&SRETRY=0},
   volume = {19},
   year = {2007}
}

TY  - JOUR
ID  - 727506
AU  - Pohanka, Miroslav - Skládal, Petr
PY  - 2007
TI  - Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor
JF  - Electroanalysis
VL  - 19
IS  - 23
SP  - 2507-2512
EP  - 2507-2512
PB  - Wiley-VCH Verlag GmbH
SN  - 10400397
N1  - DOI 10.1002/elan.200704003
KW  - Amperometric biosensor
KW  - Screen-printed sensor
KW  - Immunoassay
KW  - Francisella tularensis
UR  - http://www3.interscience.wiley.com/cgi-bin/abstract/116320027/ABSTRACT?CRETRY=1&SRETRY=0
N2  - Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.
ER  -

POHANKA, Miroslav and Petr SKLÁDAL. Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2007, vol.~19, No~23, p.~2507-2512. ISSN~1040-0397.

Detailed Information on Publication Record