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@article{727513,
author = {Pohanka, Miroslav and Skládal, Petr},
article_location = {Prague},
article_number = {4},
keywords = {Piezoelectric sensor; Quartz crystal microbalance; Immunoassay},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Piezoelectric Immunosensor for the Direct and Rapid Detection of Francisella tularensis},
url = {http://www.cssm.info/priloha/fm2007_325.pdf},
volume = {52},
year = {2007}
}
TY - JOUR
ID - 727513
AU - Pohanka, Miroslav - Skládal, Petr
PY - 2007
TI - Piezoelectric Immunosensor for the Direct and Rapid Detection of Francisella tularensis
JF - Folia Microbiologica
VL - 52
IS - 4
SP - 325-330
EP - 325-330
PB - Czechoslovak Society for Microbiology
SN - 00155632
KW - Piezoelectric sensor
KW - Quartz crystal microbalance
KW - Immunoassay
UR - http://www.cssm.info/priloha/fm2007_325.pdf
N2 - A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor included mouse polyclonal antibody immobilized through the layer of protein A covalently linked to the gold electrode of the sensor. Thus obtained immunosensor was able to detect F. tularensis with the limit of detection 105 CFU/mL with a typical measuring cycle under 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
ER -
POHANKA, Miroslav a Petr SKLÁDAL. Piezoelectric Immunosensor for the Direct and Rapid Detection of Francisella tularensis. \textit{Folia Microbiologica}. Prague: Czechoslovak Society for Microbiology, 2007, roč.~52, č.~4, s.~325-330. ISSN~0015-5632.
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