Characterization of Staphylococcus sp. deposited in the Czech
Collection of Microorganisms

a 2007

Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms

NOVÁKOVÁ, Dana, Pavel ŠVEC a Ivo SEDLÁČEK

Základní údaje

Originální název

Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms

Název česky

Charakterizace kmenů Staphylococcus sp. deponovaných v České sbírce mikroorganismů

Autoři

NOVÁKOVÁ, Dana (203 Česká republika, garant), Pavel ŠVEC (203 Česká republika) a Ivo SEDLÁČEK (203 Česká republika)

Vydání

Proceedings of the 11th International Conference on Culture Collections, 2007

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/07:00023007

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-3-00-022417-1

Klíčová slova anglicky

Staphylococcus sp.; ribotyping; whole-cell protein analysis; rep-PCR

Štítky

rep-PCR, ribotyping, Staphylococcus sp., whole-cell protein analysis

Změněno: 24. 3. 2010 13:12, doc. RNDr. Pavel Švec, Ph.D.

Anotace

ORIG CZ

V originále

The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting. EcoRI ribotype patterns revealed more species specific clusters than HindIII profiles in these strains. And finally, two pairs of analysed strains showed high similarity to each other by using all methods. They were clearly separated from all validly described staphylococcal species and probably represent new taxons of this genera. Our results show that applied methods are suitable for classification of tested Staphylococcus spp. and that their combination is a good polyphasic approach applicable for identification of staphylococci. This study was supported by projects ns. MSM0021622416 and FRVS G4 2658.

Česky

Charakterizace kmenů Staphylococcus sp. pomocí metody ribotypizace, analýzy celkových buněčných proteinů a (GTG)5-PCR.

Návaznosti

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

NOVÁKOVÁ, Dana, Pavel ŠVEC a Ivo SEDLÁČEK. Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms. In Proceedings of the 11th International Conference on Culture Collections. 2007. ISBN 978-3-00-022417-1.

@proceedings{744238,
   author = {Nováková, Dana and Švec, Pavel and Sedláček, Ivo},
   booktitle = {Proceedings of the 11th International Conference on Culture Collections},
   keywords = {Staphylococcus sp.; ribotyping; whole-cell protein analysis; rep-PCR},
   language = {eng},
   isbn = {978-3-00-022417-1},
   title = {Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms},
   year = {2007}
}

TY  - CONF
ID  - 744238
AU  - Nováková, Dana - Švec, Pavel - Sedláček, Ivo
PY  - 2007
TI  - Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms
SN  - 9783000224171
KW  - Staphylococcus sp.
KW  - ribotyping
KW  - whole-cell protein analysis
KW  - rep-PCR
N2  - The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, API Zym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruently separated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in several strains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR fingerprinting. EcoRI ribotype patterns revealed more species specific clusters than HindIII profiles in these strains. And finally, two pairs of analysed strains showed high similarity to each other by using all methods. They were clearly separated from all validly described staphylococcal species and probably represent new taxons of this genera. Our results show that applied methods are suitable for classification of tested Staphylococcus spp. and that their combination is a good polyphasic approach applicable for identification of staphylococci. This study was supported by projects ns. MSM0021622416 and FRVS G4 2658.
ER  -

NOVÁKOVÁ, Dana, Pavel ŠVEC a Ivo SEDLÁČEK. Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms. In \textit{Proceedings of the 11th International Conference on Culture Collections}. 2007. ISBN~978-3-00-022417-1.

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