Další formáty:
BibTeX
LaTeX
RIS
@article{745052, author = {Harničarová, Andrea and Kozubek, Stanislav and Pacherník, Jiří and Krejčí, Jana and Bártová, Eva}, article_number = {20}, keywords = {Chromosome structure; c-myc gene; Gene expression; RNA-FISH, Differentiation}, issn = {0014-4827}, journal = {Exp Cell Res.}, title = {Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.}, volume = {312}, year = {2006} }
TY - JOUR ID - 745052 AU - Harničarová, Andrea - Kozubek, Stanislav - Pacherník, Jiří - Krejčí, Jana - Bártová, Eva PY - 2006 TI - Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells. JF - Exp Cell Res. VL - 312 IS - 20 PB - Elsevier SN - 00144827 KW - Chromosome structure KW - c-myc gene KW - Gene expression KW - RNA-FISH, Differentiation N2 - Using sequential RNA-DNA fluorescence in situ hybridization, the nuclear arrangement of both the active and inactive c-myc gene as well as its transcription was investigated in colon cancer HT-29 cells induced to differentiate into enterocytes. Cytogenetic studies revealed the presence of two chromosomes 8 in HT-29 cells, of which the one containing c-myc gene amplicons was substantially larger and easily distinguished from the normal chromosome. This observation enabled detection of both activity and nuclear localization of c-myc genes in single cells and in individual chromosome territories. Similar transcriptional activity of the c-myc gene was observed in both the normal and derivative chromosome 8 territories showing no influence of the amplification on the c-myc gene expression. Our experiments demonstrate strikingly specific nuclear and territorial arrangements of active genes as compared with inactive ones: on the periphery of their territories facing to the very central region of the cell nucleus. Nuclear arrangement of c-myc genes and transcripts was conserved during cell differentiation and, therefore, independent of the level of differentiation-specific c-myc gene expression. However, after the induction of differentiation, a more internal territorial location was found for the single copy c-myc gene of normal chromosome 8, while amplicons conserved their territorial topography. ER -
HARNIČAROVÁ, Andrea. Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells. \textit{Exp Cell Res.}. Elsevier, 2006, roč.~312, č.~20, 4019-4035. ISSN~0014-4827.
|