Lymphocyte showing the phenotype of regulatory T-cells
infiltrate colon carcinoma

a 2007

Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ondřej SLABÝ, Ilona KOCÁKOVÁ et. al.

Basic information

Original name

Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma

Name in Czech

Lymfocyty vykazujíci fenotyp T-regulačných buněk infiltrují karcinom střeva

Authors

GARAJOVÁ, Ingrid (703 Slovakia), Pavel FABIAN (203 Czech Republic), Rudolf NENUTIL (203 Czech Republic), Ondřej SLABÝ (203 Czech Republic), Ilona KOCÁKOVÁ (203 Czech Republic), Peter GRELL (703 Slovakia), Zina HANZELKOVÁ (203 Czech Republic), Marek SVOBODA (203 Czech Republic) and Rostislav VYZULA (203 Czech Republic, guarantor)

Edition

Abstract book. 2007

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/07:00019123

Organization unit

Faculty of Medicine

Keywords in English

regulatory T-cells; colon carcinoma

Abstract

ORIG CZ

V originále

We have previously shown that colon carcinoma (CC) is infiltrated by FOXP3-positive lymphocytes. Although the transcription factor FOXP3 is claimed to be specific for regulatory T (TREG) cells, the canonical TREG population is known to show the CD4+ CD25+ (FOXP3+) immunophenotype. The aim of the current study was therefore to investigate whether FOXP3+ cells infiltrating CC coexpress indeed CD4 and CD25 molecules. Double-staining experiments were therefore performed on serial sections obtained from formalin-fixed, paraffin-embedded specimens of nine cases of CC. The CC infiltrate included numerous CD4+ as well as CD25+ cells; moreover CD4+ cells harboured both FOXP3+ and FOXP3- lymphocytes; similarly, CD25+ cells included both FOXP3+ and FOXP3- lymphocytes. Nevertheless, all the FOXP3+ cells showed simultaneously CD4-positivity; similarly, all the FOXP3+ cells showed simultaneously CD25-positivity. Thus, the FOXP3+ lymphocytes infiltrating CC are characterized by the coexpression of both CD4 and CD25 molecules, and show therefore an immunophenotype overlapping with that of standard CD4+CD25+FOXP3+ TREG cells. The presence of lymphocytes resembling TREG cells within the microenvironment of CC can explain why the CC tumour mass, despite being also infiltrated by cytolytic anti-tumour CD8+/CD4+ lymphocytes, shows however the well known capability to undergo fatal progression. Acknowledgment: This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.: NR/9076-4.

In Czech

Publikace je pouze v anglickém jazyku.

Links

NR9076, research and development project

Name: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku

Citovat

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ondřej SLABÝ, Ilona KOCÁKOVÁ, Peter GRELL, Zina HANZELKOVÁ, Marek SVOBODA and Rostislav VYZULA. Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma. In Abstract book. 2007.

@proceedings{746221,
   author = {Garajová, Ingrid and Fabian, Pavel and Nenutil, Rudolf and Slabý, Ondřej and Kocáková, Ilona and Grell, Peter and Hanzelková, Zina and Svoboda, Marek and Vyzula, Rostislav},
   booktitle = {Abstract book.},
   keywords = {regulatory T-cells; colon carcinoma},
   language = {eng},
   title = {Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma},
   year = {2007}
}

TY  - CONF
ID  - 746221
AU  - Garajová, Ingrid - Fabian, Pavel - Nenutil, Rudolf - Slabý, Ondřej - Kocáková, Ilona - Grell, Peter - Hanzelková, Zina - Svoboda, Marek - Vyzula, Rostislav
PY  - 2007
TI  - Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma
KW  - regulatory T-cells
KW  - colon carcinoma
N2  - We have previously shown that colon carcinoma (CC) is infiltrated by FOXP3-positive lymphocytes. Although the transcription factor FOXP3 is claimed to be specific for regulatory T (TREG) cells, the canonical TREG population is known to show the CD4+ CD25+ (FOXP3+) immunophenotype. The aim of the current study was therefore to investigate whether FOXP3+ cells infiltrating CC coexpress indeed CD4 and CD25 molecules. Double-staining experiments were therefore performed on serial sections obtained from formalin-fixed, paraffin-embedded specimens of nine cases of CC. The CC infiltrate included numerous CD4+ as well as CD25+ cells; moreover CD4+ cells harboured both FOXP3+ and FOXP3- lymphocytes; similarly, CD25+ cells included both FOXP3+ and FOXP3- lymphocytes. Nevertheless, all the FOXP3+ cells showed simultaneously CD4-positivity; similarly, all the FOXP3+ cells showed simultaneously CD25-positivity. Thus, the FOXP3+ lymphocytes infiltrating CC are characterized by the coexpression of both CD4 and CD25 molecules, and show therefore an immunophenotype overlapping with that of standard CD4+CD25+FOXP3+ TREG cells. The presence of lymphocytes resembling TREG cells within the microenvironment of CC can explain why the CC tumour mass, despite being also infiltrated by cytolytic anti-tumour CD8+/CD4+ lymphocytes, shows however the well known capability to undergo fatal progression. Acknowledgment: This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.: NR/9076-4.
ER  -

Detailed Information on Publication Record