Electroanalytical techniques for determination of flavonoids

PRŮŠA, Richard, Vojtěch ADAM, Pavel HANUŠTIAK, Petr HODEK, Jiří KUKAČKA, Marie STIBOROVÁ, Miroslava BEKLOVÁ, Libuše TRNKOVÁ, Aleš HORNA a René KIZEK. Electroanalytical techniques for determination of flavonoids. In EuroMedlab Amsterdam 2007. Amsterdam, The Netherlands: International Federation of Clinical Chemistry and Laboratory Medicine, 2007, s. 204-204. ISSN 1437-8523.

Základní údaje

• Originální název: Electroanalytical techniques for determination of flavonoids
• Název česky: Elektrochemické techniky pro stanovení flavonoidů
• Název anglicky: Electroanalytical techniques for determination of flavonoids
• Autoři: PRŮŠA, Richard, Vojtěch ADAM, Pavel HANUŠTIAK, Petr HODEK, Jiří KUKAČKA, Marie STIBOROVÁ, Miroslava BEKLOVÁ, Libuše TRNKOVÁ, Aleš HORNA a René KIZEK.
• Vydání: Amsterdam, The Netherlands, EuroMedlab Amsterdam 2007, od s. 204-204, 1 s. 2007.
• Nakladatel: International Federation of Clinical Chemistry and Laboratory Medicine

Další údaje

• Originální jazyk: čeština
• Typ výsledku: Stať ve sborníku
• Obor: 10405 Electrochemistry
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Organizační jednotka: Přírodovědecká fakulta
• ISSN: 1437-8523
• Klíčová slova anglicky: flavonoids; voltammetry; cytochroms
• Štítky: cytochroms, flavonoids, Voltammetry

Anotace

Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge or by a pyrane or pyrone ring. Flavonoids have been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser. Results. Due to high abundance of flavonoids in human diet, we decided not only to optimize the square wave voltammetry for the detection of quercetin, quercitrin, rutin, chrysin and diosmin but also to examine them as potential inducers of cytochrome(s). Based on the results obtained, the most suitable conditions for determination of the flavonoids were as follows: frequency 180 Hz, step potential 1.95 mV/s and phosphate buffer of pH 7 as supporting electrolyte. The detection limits of the flavonoids were about tens of nM. After that we examined the certain flavonoids as potential inducers of cytochrome(s) P450. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism in the body. Conclusions. Electrochemical techniques represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without harming or killing of living organism in comparison with the commonly methods used for investigation of flavonoids.

Anotace anglicky

Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge or by a pyrane or pyrone ring. Flavonoids have been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser. Results. Due to high abundance of flavonoids in human diet, we decided not only to optimize the square wave voltammetry for the detection of quercetin, quercitrin, rutin, chrysin and diosmin but also to examine them as potential inducers of cytochrome(s). Based on the results obtained, the most suitable conditions for determination of the flavonoids were as follows: frequency 180 Hz, step potential 1.95 mV/s and phosphate buffer of pH 7 as supporting electrolyte. The detection limits of the flavonoids were about tens of nM. After that we examined the certain flavonoids as potential inducers of cytochrome(s) P450. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism in the body. Conclusions. Electrochemical techniques represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without harming or killing of living organism in comparison with the commonly methods used for investigation of flavonoids.