Electroanalytical techniques for determination of flavonoids

PRŮŠA, Richard, Vojtěch ADAM, Pavel HANUŠTIAK, Petr HODEK, Jiří KUKAČKA, Marie STIBOROVÁ, Miroslava BEKLOVÁ, Libuše TRNKOVÁ, Aleš HORNA and René KIZEK. Electroanalytical techniques for determination of flavonoids. In EuroMedlab Amsterdam 2007. Amsterdam, The Netherlands: International Federation of Clinical Chemistry and Laboratory Medicine, 2007, p. 204-204. ISSN 1437-8523.

Basic information

Original name

Electroanalytical techniques for determination of flavonoids

Name in Czech

Elektrochemické techniky pro stanovení flavonoidů

Name (in English)

Electroanalytical techniques for determination of flavonoids

Authors

PRŮŠA, Richard, Vojtěch ADAM, Pavel HANUŠTIAK, Petr HODEK, Jiří KUKAČKA, Marie STIBOROVÁ, Miroslava BEKLOVÁ, Libuše TRNKOVÁ, Aleš HORNA and René KIZEK

Edition

Amsterdam, The Netherlands, EuroMedlab Amsterdam 2007, p. 204-204, 1 pp. 2007

Publisher

International Federation of Clinical Chemistry and Laboratory Medicine

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Other information

Language

Czech

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

ISSN

Keywords in English

flavonoids; voltammetry; cytochroms

Tags

cytochroms, flavonoids, Voltammetry

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Abstract

V originále

Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge or by a pyrane or pyrone ring. Flavonoids have been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser. Results. Due to high abundance of flavonoids in human diet, we decided not only to optimize the square wave voltammetry for the detection of quercetin, quercitrin, rutin, chrysin and diosmin but also to examine them as potential inducers of cytochrome(s). Based on the results obtained, the most suitable conditions for determination of the flavonoids were as follows: frequency 180 Hz, step potential 1.95 mV/s and phosphate buffer of pH 7 as supporting electrolyte. The detection limits of the flavonoids were about tens of nM. After that we examined the certain flavonoids as potential inducers of cytochrome(s) P450. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism in the body. Conclusions. Electrochemical techniques represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without harming or killing of living organism in comparison with the commonly methods used for investigation of flavonoids.

In English

Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge or by a pyrane or pyrone ring. Flavonoids have been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser. Results. Due to high abundance of flavonoids in human diet, we decided not only to optimize the square wave voltammetry for the detection of quercetin, quercitrin, rutin, chrysin and diosmin but also to examine them as potential inducers of cytochrome(s). Based on the results obtained, the most suitable conditions for determination of the flavonoids were as follows: frequency 180 Hz, step potential 1.95 mV/s and phosphate buffer of pH 7 as supporting electrolyte. The detection limits of the flavonoids were about tens of nM. After that we examined the certain flavonoids as potential inducers of cytochrome(s) P450. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism in the body. Conclusions. Electrochemical techniques represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without harming or killing of living organism in comparison with the commonly methods used for investigation of flavonoids.