Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero.

KŘÍŽ, Vítězslav, Jaroslav MAREŠ, Parri WENTZEL, Nina FUNA, Gabriela ČALOUNOVÁ, Xiao-Qun ZHANG, Karin FORSBERG-NILSSON, Maud FORSBERG a Michael WELSH. Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero. Developmental dynamics. United States: Wiley-Liss, 2007, roč. 236, č. 9, s. 2485-2492. ISSN 1058-8388. Dostupné z: https://dx.doi.org/10.1002/dvdy.21257.

Základní údaje

• Originální název: Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero.
• Název česky: Shb null allele is inherited with a transmission ratio distortion and causes reduced viability in utero.
• Autoři: KŘÍŽ, Vítězslav (203 Česká republika, garant, domácí), Jaroslav MAREŠ (203 Česká republika), Parri WENTZEL (752 Švédsko), Nina FUNA (752 Švédsko), Gabriela ČALOUNOVÁ (203 Česká republika), Xiao-Qun ZHANG (156 Čína), Karin FORSBERG-NILSSON (752 Švédsko), Maud FORSBERG (752 Švédsko) a Michael WELSH (840 Spojené státy).
• Vydání: Developmental dynamics, United States, Wiley-Liss, 2007, 1058-8388.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: Genetika a molekulární biologie
• Stát vydavatele: Spojené státy
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 3.084
• Kód RIV: RIV/00216224:14310/07:00050891
• Organizační jednotka: Přírodovědecká fakulta
• Doi: http://dx.doi.org/10.1002/dvdy.21257
• UT WoS: 000249539700012
• Klíčová slova anglicky: SHB; knockout; ovulation; malformations; viability in utero; transmission ratio distortion
• Štítky: AKR, knockout, malformations, ovulation, rivok, SHB, transmission ratio distortion, viability in utero
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

SHB is an Src homology 2 domain-containing adapter protein that has been found to be involved in numerous cellular responses. We have generated an Shb knockout mouse. No Shb-/- pups or embryos were obtained on the C57Bl6 background, indicating an early defect as a consequence of Shb- gene inactivation on this genetic background. Breeding heterozygotes for Shb gene inactivation (Shb+/-) on a mixed genetic background (FVB/C57Bl6/129Sv) reveals a distorted transmission ratio of the null allele with reduced numbers of Shb+/+ and Shb-/- animals, but increased number of Shb+/- animals. The Shb- allele is associated with various forms of malformations, explaining the relative reduction in the number of Shb-/- offspring. Shb-/- animals that were born were viable, fertile, and showed no obvious defects. However, Shb+/- female mice ovulated preferentially Shb- oocytes explaining the reduced frequency of Shb+/+ mice. Our study suggests a role of SHB during reproduction and development.

Anotace česky

SHB is an Src homology 2 domain-containing adapter protein that has been found to be involved in numerous cellular responses. We have generated an Shb knockout mouse. No Shb-/- pups or embryos were obtained on the C57Bl6 background, indicating an early defect as a consequence of Shb- gene inactivation on this genetic background. Breeding heterozygotes for Shb gene inactivation (Shb+/-) on a mixed genetic background (FVB/C57Bl6/129Sv) reveals a distorted transmission ratio of the null allele with reduced numbers of Shb+/+ and Shb-/- animals, but increased number of Shb+/- animals. The Shb- allele is associated with various forms of malformations, explaining the relative reduction in the number of Shb-/- offspring. Shb-/- animals that were born were viable, fertile, and showed no obvious defects. However, Shb+/- female mice ovulated preferentially Shb- oocytes explaining the reduced frequency of Shb+/+ mice. Our study suggests a role of SHB during reproduction and development.