| STRAŠÁK, Luděk, Eva BÁRTOVÁ, Lukáš FOJT, Stanislav KOZUBEK a Vladimír VETTERL. Effect of UV irradiation on chromosome and chromatin of HT-29 cells. In BES 2007 Toulouse, Book of Abstracts. Toulouse: The Bioelectrochemical Society, 2007, s. 209. |
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@inproceedings{749657,
author = {Strašák, Luděk and Bártová, Eva and Fojt, Lukáš and Kozubek, Stanislav and Vetterl, Vladimír},
address = {Toulouse},
booktitle = {BES 2007 Toulouse, Book of Abstracts},
keywords = {UV irradiation; HT-29 cells},
language = {eng},
location = {Toulouse},
pages = {209-209},
publisher = {The Bioelectrochemical Society},
title = {Effect of UV irradiation on chromosome and chromatin of HT-29 cells},
year = {2007}
}
TY - JOUR
ID - 749657
AU - Strašák, Luděk - Bártová, Eva - Fojt, Lukáš - Kozubek, Stanislav - Vetterl, Vladimír
PY - 2007
TI - Effect of UV irradiation on chromosome and chromatin of HT-29 cells
PB - The Bioelectrochemical Society
CY - Toulouse
KW - UV irradiation
KW - HT-29 cells
N2 - We used HT-29 cells to study effects of UV irradiation on cell level. The effect on cytoskeleton and high-ordered chromatin structure were analyzed after 48 hours of UV exposure. The cells were cultivated at 37 C in humidified atmosphere containing 5% CO2. We used FISH (fluorescence in-situ hybridization) methodology to quantify UV irradiation effect. We estimated F-actin by FITC-conjugated Phalloidin and mitochondria by MitoTracker. Images of cytoskeleton and genetic loci were acquired using confocal microscopy and analysis was performed by FISH 2.0 software. Slight morphological changes of F-actin filaments and mitochondria were observed in affected cells and nuclear condensation was found. We found changes in affected cells.
ER -
STRAŠÁK, Luděk, Eva BÁRTOVÁ, Lukáš FOJT, Stanislav KOZUBEK a Vladimír VETTERL. Effect of UV irradiation on chromosome and chromatin of HT-29 cells. In \textit{BES 2007 Toulouse, Book of Abstracts}. Toulouse: The Bioelectrochemical Society, 2007, s.~209.
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