Sensitive detection of oligonucleotides and their adsorption on
amalgam and titanium surface.

J 2007

Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.

VETTERL, Vladimír; Jiří VANĚK; Stanislav HASOŇ a Raimo SILVENNOINEN

Základní údaje

Originální název

Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.

Název česky

Citlivá detekce oligonucleotidů a jejich adsorpce na povrchu amalgámu a titánu.

Autoři

VETTERL, Vladimír; Jiří VANĚK; Stanislav HASOŇ a Raimo SILVENNOINEN

Vydání

European Biophysics Journal, Berlin Heidelberg, Springer-Verlag, 2007, 1432-1017

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

amalgam electrodes; oligodeoxynucleotides detection

Štítky

amalgam electrodes, oligodeoxynucleotides detection

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 3. 2008 16:57, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

ORIG CZ

V originále

The application of a gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper ions is described. Our result shows that the sensitive detection of different ODNs can be also performed at the platinum amalgam-alloy electrode (PtAE) contrary to a low sensitivity at the silver amalgam-alloy electrode (AgAE).Electrochemical impedance spectroscopy (EIS), voltammetry, AFM, and DOE was used for the investigations of adsorption of oligonucleotides on the titanium surface and the effect of mechanical (polished, etched and/or gritted surface) and chemical modification of titanium surface was studied. From the EIS, voltammetric and optical measurements the conclusions of the effect of solvent and physical or chemical modification of titanium surface on the purine (AAG)12 and pyrimidine (TTC)12 oligonucleotide adsorption was delivered.

Česky

Je popsáno použití amalgamových elektrod k citlivé detekci oligodeoxynukleotidů.

Návaznosti

1M0528, projekt VaV

Název: Stomatologické výzkumné centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Stomatologické výzkumné centrum

Citovat

VETTERL, Vladimír; Jiří VANĚK; Stanislav HASOŇ a Raimo SILVENNOINEN. Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface. European Biophysics Journal. Berlin Heidelberg: Springer-Verlag, 2007, roč. 36, č. 2007, s. 204. ISSN 1432-1017.

@article{749694,
   author = {Vetterl, Vladimír and Vaněk, Jiří and Hasoň, Stanislav and Silvennoinen, Raimo},
   article_location = {Berlin Heidelberg},
   article_number = {2007},
   keywords = {amalgam electrodes; oligodeoxynucleotides detection},
   language = {eng},
   issn = {1432-1017},
   journal = {European Biophysics Journal},
   title = {Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.},
   volume = {36},
   year = {2007}
}

TY  - JOUR
ID  - 749694
AU  - Vetterl, Vladimír - Vaněk, Jiří - Hasoň, Stanislav - Silvennoinen, Raimo
PY  - 2007
TI  - Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface.
JF  - European Biophysics Journal
VL  - 36
IS  - 2007
SP  - 204
EP  - 204
PB  - Springer-Verlag
SN  - 14321017
KW  - amalgam electrodes
KW  - oligodeoxynucleotides detection
N2  - The application of a gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper ions is described. Our result shows that the sensitive detection of different ODNs can be also performed at the platinum amalgam-alloy electrode (PtAE) contrary to a low sensitivity at the silver amalgam-alloy electrode (AgAE).Electrochemical impedance spectroscopy (EIS), voltammetry, AFM, and DOE was used for the investigations of adsorption of oligonucleotides on the titanium surface and the effect of mechanical (polished, etched and/or gritted surface) and chemical modification of titanium surface was studied. From the EIS, voltammetric and optical measurements the conclusions of the effect of solvent and physical or chemical modification of titanium surface on the purine (AAG)12 and pyrimidine (TTC)12 oligonucleotide adsorption was delivered.
ER  -

VETTERL, Vladimír; Jiří VANĚK; Stanislav HASOŇ a Raimo SILVENNOINEN. Sensitive detection of oligonucleotides and their adsorption on amalgam and titanium surface. \textit{European Biophysics Journal}. Berlin Heidelberg: Springer-Verlag, 2007, roč.~36, č.~2007, s.~204. ISSN~1432-1017.

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