EFFECTS OF DEFINED PAH MIXTURES IN VARIOUS IN VITRO BIOASSAYS

HILSCHEROVÁ, Klára, Martin BENÍŠEK a Tereza ŠÍDLOVÁ. EFFECTS OF DEFINED PAH MIXTURES IN VARIOUS IN VITRO BIOASSAYS. In Abstract Book 10 Jahre SETAC-GLB. 2007.

Základní údaje

• Originální název: EFFECTS OF DEFINED PAH MIXTURES IN VARIOUS IN VITRO BIOASSAYS
• Název česky: Účinky modelových směsí PAH v různých in vitro biotestech
• Název anglicky: EFFECTS OF DEFINED PAH MIXTURES IN VARIOUS IN VITRO BIOASSAYS
• Autoři: HILSCHEROVÁ, Klára, Martin BENÍŠEK a Tereza ŠÍDLOVÁ.
• Vydání: Abstract Book 10 Jahre SETAC-GLB, 2007.

Další údaje

• Typ výsledku: Prezentace na konferencích
• Utajení: není předmětem státního či obchodního tajemství
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: mixtures, PAH,
• Štítky: mixtures, PAH,
• Příznaky: Mezinárodní význam

Anotace

Most of toxicity studies with environmental pollutants focus only on single agents whereas organisms are exposed to complex mixtures in the environment. These mixtures often contain compounds able to activate aryl hydrocarbon receptor (AhR) or act as anti/estrogenic or anti/androgenic chemicals. Between these, most frequent are polychlorinated dioxins and furans, polychlorinated biphenyls, pesticides and also polycyclic aromatic hydrocarbons (PAHs). Environmental mixtures can contain many of these components and analyses of the resulting interactions can be exceedingly complex. Thus, defined mixtures of two or more compounds are widely used to study toxic effects of mixtures. In this study we measured potency of selected PAHs and their binary and ternary mixtures to act through some specific modes of action (such as AhR activity, antiandrogenicity etc.) using in vitro bioassays based on mammalian cell lines or yeast-based assays. Studied PAHs, namely benz[a]anthracene (BaA), benzo[a]pyrene (BaP), dibenz[a,h]anthracene (DB[a,h]A] and fluoranthene (Fl) were tested either individually or as binary or ternary mixtures and obtained results of mixtures were compared with concentration addition model and suitability of yeast assays for PAHs mixture toxicity testing was evaluated. This study

Anotace anglicky

Most of toxicity studies with environmental pollutants focus only on single agents whereas organisms are exposed to complex mixtures in the environment. These mixtures often contain compounds able to activate aryl hydrocarbon receptor (AhR) or act as anti/estrogenic or anti/androgenic chemicals. Between these, most frequent are polychlorinated dioxins and furans, polychlorinated biphenyls, pesticides and also polycyclic aromatic hydrocarbons (PAHs). Environmental mixtures can contain many of these components and analyses of the resulting interactions can be exceedingly complex. Thus, defined mixtures of two or more compounds are widely used to study toxic effects of mixtures. In this study we measured potency of selected PAHs and their binary and ternary mixtures to act through some specific modes of action (such as AhR activity, antiandrogenicity etc.) using in vitro bioassays based on mammalian cell lines or yeast-based assays. Studied PAHs, namely benz[a]anthracene (BaA), benzo[a]pyrene (BaP), dibenz[a,h]anthracene (DB[a,h]A] and fluoranthene (Fl) were tested either individually or as binary or ternary mixtures and obtained results of mixtures were compared with concentration addition model and suitability of yeast assays for PAHs mixture toxicity testing was evaluated.

Návaznosti

• GP525/05/P160, projekt VaV: Název: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů

Investor: Grantová agentura ČR, In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů