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@article{761324,
author = {Křížková, Soňa and Ryant, Pavel and Kryštofová, Olga and Adam, Vojtěch and Galiová, Michaela and Beklová, Miroslava and Babula, Petr and Kaiser, Jozef and Novotný, Karel and Novotný, Jan and Liška, Miroslav and Malina, Radomír and Zehnálek, Josef and Hubálek, Jaromír and Havel, Ladislav and Kizek, René},
article_number = {1},
keywords = {silver; heavy metals; plant biosensor; sensors; biochemical marker},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution},
volume = {8},
year = {2008}
}
TY - JOUR
ID - 761324
AU - Křížková, Soňa - Ryant, Pavel - Kryštofová, Olga - Adam, Vojtěch - Galiová, Michaela - Beklová, Miroslava - Babula, Petr - Kaiser, Jozef - Novotný, Karel - Novotný, Jan - Liška, Miroslav - Malina, Radomír - Zehnálek, Josef - Hubálek, Jaromír - Havel, Ladislav - Kizek, René
PY - 2008
TI - Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution
JF - Sensors
VL - 8
IS - 1
SP - 445-463
EP - 445-463
SN - 14248220
KW - silver
KW - heavy metals
KW - plant biosensor
KW - sensors
KW - biochemical marker
N2 - The aim of this work is to investigate sunflower plants response on stress induced by silver(I) ions. The sunflower plants were exposed to silver(I) ions (0, 0.1, 0.5, and 1 mM) for 96 h. Primarily we aimed our attention to observation of basic physiological parameters. We found that the treated plants embodied growth depression, coloured changes and lack root hairs. Using of autofluorescence of anatomical structures, such as lignified cell walls, it was possible to determine the changes of important shoot and root structures, mainly vascular bungles and development of secondary thickening. The differences in vascular bundles organisation, parenchymatic pith development in the root centre and the reduction of phloem part of vascular bundles were well observable. Moreover with increasing silver(I) ions concentration the vitality of rhizodermal cells declined; rhizodermal cells early necrosed and were replaced by the cells of exodermis. Further we employed laser induced breakdown spectroscopy for determination of spatial distribution of silver(I) ions in tissues of the treated plants. The Ag is accumulated mainly in near-root part of the sample. Moreover basic biochemical indicators of environmental stress were investigated. The total content of proteins expressively decreased with increasing silver(I) ions dose and the time of the treatment. As we compare the results obtained by protein analysis-the total protein contents in shoot as well as root parts-we can assume on the transport of the proteins from the roots to shoots. This phenomenon can be related with the cascade of processes connecting with photosynthesis. The second biochemical parameter, which we investigated, was urease activity. If we compared the activity in treated plants with control, we found out that presence of silver(I) ions markedly enhanced the activity of urease at all applied doses of this toxic metal. Finally we studied the effect of silver(I) ions on activity of urease in in vitro conditions.
ER -
KŘÍŽKOVÁ, Soňa, Pavel RYANT, Olga KRYŠTOFOVÁ, Vojtěch ADAM, Michaela GALIOVÁ, Miroslava BEKLOVÁ, Petr BABULA, Jozef KAISER, Karel NOVOTNÝ, Jan NOVOTNÝ, Miroslav LIŠKA, Radomír MALINA, Josef ZEHNÁLEK, Jaromír HUBÁLEK, Ladislav HAVEL a René KIZEK. Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as Bioindicators of Environmental Pollution. \textit{Sensors}. 2008, roč.~8, č.~1, s.~445-463. ISSN~1424-8220.
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