aro Mutations in Salmonella enterica Cause Defects in Cell Wall
and Outer Membrane Integrity

J 2008

aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

ŠEBKOVÁ, Alena; Daniela KARASOVÁ; Magdaléna CRHÁNOVÁ; Eva BUDINSKÁ; Ivan RYCHLÍK et al.

Základní údaje

Originální název

aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity

Název česky

aro mutace u Salmonella enterica způsobují defekty buněčné stěny a zevní membránové integrity

Autoři

ŠEBKOVÁ, Alena; Daniela KARASOVÁ; Magdaléna CRHÁNOVÁ; Eva BUDINSKÁ a Ivan RYCHLÍK

Vydání

JOURNAL OF BACTERIOLOGY, American Society for Microbiology, 2008, 0021-9193

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.636

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/08:00050960

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1128/JB.00053-08

UT WoS

000255202100008

Klíčová slova anglicky

aro mutant; Salmonella enterica; microarray; gene expression; plasmid

Příznaky

Mezinárodní význam

Změněno: 18. 4. 2012 11:08, Mgr. Michal Petr

Anotace

V originále

In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.

Návaznosti

LC06030, projekt VaV

Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

ŠEBKOVÁ, Alena; Daniela KARASOVÁ; Magdaléna CRHÁNOVÁ; Eva BUDINSKÁ a Ivan RYCHLÍK. aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity. JOURNAL OF BACTERIOLOGY. American Society for Microbiology, 2008, roč. 190, č. 9, s. 3155-3160. ISSN 0021-9193. Dostupné z: https://doi.org/10.1128/JB.00053-08.

@article{764213,
   author = {Šebková, Alena and Karasová, Daniela and Crhánová, Magdaléna and Budinská, Eva and Rychlík, Ivan},
   article_number = {9},
   doi = {https://doi.org/10.1128/JB.00053-08},
   keywords = {aro mutant; Salmonella enterica; microarray; gene expression; plasmid},
   language = {eng},
   issn = {0021-9193},
   journal = {JOURNAL OF BACTERIOLOGY},
   title = {aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity},
   url = {http://jb.asm.org/cgi/content/abstract/190/9/3155},
   volume = {190},
   year = {2008}
}

TY  - JOUR
ID  - 764213
AU  - Šebková, Alena - Karasová, Daniela - Crhánová, Magdaléna - Budinská, Eva - Rychlík, Ivan
PY  - 2008
TI  - aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity
JF  - JOURNAL OF BACTERIOLOGY
VL  - 190
IS  - 9
SP  - 3155-3160
EP  - 3155-3160
PB  - American Society for Microbiology
SN  - 00219193
KW  - aro mutant
KW  - Salmonella enterica
KW  - microarray
KW  - gene expression
KW  - plasmid
UR  - http://jb.asm.org/cgi/content/abstract/190/9/3155
L2  - http://jb.asm.org/cgi/content/abstract/190/9/3155
N2  - In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA, aroD, and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA. To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response.
ER  -

ŠEBKOVÁ, Alena; Daniela KARASOVÁ; Magdaléna CRHÁNOVÁ; Eva BUDINSKÁ a Ivan RYCHLÍK. aro Mutations in Salmonella enterica Cause Defects in Cell Wall and Outer Membrane Integrity. \textit{JOURNAL OF BACTERIOLOGY}. American Society for Microbiology, 2008, roč.~190, č.~9, s.~3155-3160. ISSN~0021-9193. Dostupné z: https://doi.org/10.1128/JB.00053-08.

Přehled o publikaci