Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB
13064 Diffracted to Ultrahigh Resolution: Crystallization and
Preliminary Diffraction Analysis.

J 2008

Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.

STSIASPANAVA, A.; Táňa KOUDELÁKOVÁ; M. LAPKOUSKI; Martina PAVLOVÁ; Jiří DAMBORSKÝ et al.

Základní údaje

Originální název

Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.

Název česky

Krystaly z mutantu DhaA z bakterie Rhodococcus rhodochrous NCIMB 13064 : Krystalizace a difrakční analýza.

Autoři

STSIASPANAVA, A.; Táňa KOUDELÁKOVÁ; M. LAPKOUSKI; Martina PAVLOVÁ; Jiří DAMBORSKÝ a Ivana KUTÁ-SMATANOVÁ

Vydání

Acta Crystallographica Section D: Biological Crystallography, 2008, 1744-3091

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.606

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/08:00026132

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000252817400021

Klíčová slova anglicky

haloalkane dehalogenases; enzyme DhaA; crystals;

Štítky

crystals, enzyme DhaA, Haloalkane dehalogenases

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 7. 2009 08:10, Mgr. Táňa Koudeláková, Ph.D.

Anotace

ORIG CZ

V originále

The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. All protein mutants were crystallized using the sitting-drop vapour-diffusion method. The crystals of DhaA04 belonged to the orthorhombic space group P212121, while the crystals of the other two mutants DhaA14 and DhaA15 belonged to the triclinic space group P1. Native data sets were collected for the DhaA04, DhaA14 and DhaA15 mutants at beamline X11 of EMBL, DESY, Hamburg to the high resolutions of 1.30, 0.95 and 1.15 A, respectively.

Česky

Enzym DhaA z bakterie Rhodococcus rhodochrous NCIMB 13064 patří k haloalkan dehalogenázám, které katalyzují hydrlýzy haloalkán na odpovídající alkoholy.Byly zkonstruovány 3 matuanty DhaA04, DhaA14 a DhaA15.

Návaznosti

LC06010, projekt VaV

Název: Centrum biokatalýzy a biotransformací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum biokatalýzy a biotransformací

Citovat

STSIASPANAVA, A.; Táňa KOUDELÁKOVÁ; M. LAPKOUSKI; Martina PAVLOVÁ; Jiří DAMBORSKÝ a Ivana KUTÁ-SMATANOVÁ. Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis. Acta Crystallographica Section D: Biological Crystallography. 2008, F, č. 64, s. 137-140. ISSN 1744-3091.

@article{770117,
   author = {Stsiaspanava, A. and Koudeláková, Táňa and Lapkouski, M. and Pavlová, Martina and Damborský, Jiří and KutáandSmatanová, Ivana},
   article_number = {64},
   keywords = {haloalkane dehalogenases; enzyme DhaA; crystals;},
   language = {eng},
   issn = {1744-3091},
   journal = {Acta Crystallographica Section D: Biological Crystallography},
   title = {Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.},
   url = {http://loschmidt.chemi.muni.cz/peg/abstracts/acsf08.html},
   volume = {F},
   year = {2008}
}

TY  - JOUR
ID  - 770117
AU  - Stsiaspanava, A. - Koudeláková, Táňa - Lapkouski, M. - Pavlová, Martina - Damborský, Jiří - Kutá-Smatanová, Ivana
PY  - 2008
TI  - Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis.
JF  - Acta Crystallographica Section D: Biological Crystallography
VL  - F
IS  - 64
SP  - 137-140
EP  - 137-140
SN  - 17443091
KW  - haloalkane dehalogenases
KW  - enzyme DhaA
KW  - crystals;
UR  - http://loschmidt.chemi.muni.cz/peg/abstracts/acsf08.html
N2  - The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. All protein mutants were crystallized using the sitting-drop vapour-diffusion method. The crystals of DhaA04 belonged to the orthorhombic space group P212121, while the crystals of the other two mutants DhaA14 and DhaA15 belonged to the triclinic space group P1. Native data sets were collected for the DhaA04, DhaA14 and DhaA15 mutants at beamline X11 of EMBL, DESY, Hamburg to the high resolutions of 1.30, 0.95 and 1.15 A, respectively.
ER  -

STSIASPANAVA, A.; Táňa KOUDELÁKOVÁ; M. LAPKOUSKI; Martina PAVLOVÁ; Jiří DAMBORSKÝ a Ivana KUTÁ-SMATANOVÁ. Crystals of DhaA Mutants from Rhodococcus rhodochrous NCIMB 13064 Diffracted to Ultrahigh Resolution: Crystallization and Preliminary Diffraction Analysis. \textit{Acta Crystallographica Section D: Biological Crystallography}. 2008, F, č.~64, s.~137-140. ISSN~1744-3091.

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