Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA
Polymerase-beta Heterozygous Mice

J 2008

Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice

ALLEN, Diwi; Damon, C. HERBERT; Alex, C. MCMAHAN; Vladimír ROTREKL; Robert, W. SOBOL et al.

Základní údaje

Originální název

Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice

Název česky

Zvýšená hladina mutageneze samčích zárodečných buněk, získaných z myší heterozygotních pro DNA polymerázu beta

Autoři

ALLEN, Diwi; Damon, C. HERBERT; Alex, C. MCMAHAN; Vladimír ROTREKL; Robert, W. SOBOL; Samuel, H. WILSON a Christi, A. WALTER

Vydání

Biology of Reproduction, Madison, Society for the Study of Reproduction, 2008, 0006-3363

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.469

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

UT WoS

000260321300005

Klíčová slova anglicky

base excision repair DNA repair gamete biology gametogenesis lacI mutagenesis POLB spermatogenesis spermatogenic cells

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 4. 2020 00:32, Mgr. Michal Petr

Anotace

V originále

Gametes carry the DNA that will direct the development of the next generation. By compromising genetic integrity, DNA damage and mutagenesis threaten the ability of gametes to fulfill their biological function.High base excision repair (BER) activity is documented for spermatogenic cells. In vitro short-patch BER activity assays revealed that spermatogenic cell nuclear extracts obtained from Polb heterozygous mice had one third the BER activity of age-matched control mice. The spontaneous mutant frequency for mixed spermatogenic cells prepared from Polb heterozygous mice was 2-fold greater than that of wild-type controls, but no significant effect was found among the somatic tissues tested. These results demonstrate that normal POLB abundance is necessary for normal BER activity, which is critical in maintaining a low germline mutant frequency. Notably, spermatogenic cells respond differently than somatic cells to Polb haploinsufficiency.

Citovat

ALLEN, Diwi; Damon, C. HERBERT; Alex, C. MCMAHAN; Vladimír ROTREKL; Robert, W. SOBOL; Samuel, H. WILSON a Christi, A. WALTER. Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice. Biology of Reproduction. Madison: Society for the Study of Reproduction, 2008, roč. 79, č. 5, s. 824-831. ISSN 0006-3363.

@article{773200,
   author = {Allen, Diwi and Herbert, Damon, C. and McMahan, Alex, C. and Rotrekl, Vladimír and Sobol, Robert, W. and Wilson, Samuel, H. and Walter, Christi, A.},
   article_location = {Madison},
   article_number = {5},
   keywords = {base excision repair DNA repair gamete biology gametogenesis lacI mutagenesis POLB spermatogenesis spermatogenic cells},
   language = {eng},
   issn = {0006-3363},
   journal = {Biology of Reproduction},
   title = {Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice},
   volume = {79},
   year = {2008}
}

TY  - JOUR
ID  - 773200
AU  - Allen, Diwi - Herbert, Damon, C. - McMahan, Alex, C. - Rotrekl, Vladimír - Sobol, Robert, W. - Wilson, Samuel, H. - Walter, Christi, A.
PY  - 2008
TI  - Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice
JF  - Biology of Reproduction
VL  - 79
IS  - 5
SP  - 824-831
EP  - 824-831
PB  - Society for the Study of Reproduction
SN  - 00063363
KW  - base excision repair DNA repair gamete biology gametogenesis lacI mutagenesis POLB spermatogenesis spermatogenic cells
N2  - Gametes carry the DNA that will direct the development of the next generation. By compromising genetic integrity, DNA damage and mutagenesis threaten the ability of gametes to fulfill their biological function.High base excision repair (BER) activity is documented for spermatogenic cells. In vitro short-patch BER activity assays revealed that spermatogenic cell nuclear extracts obtained from Polb heterozygous mice had one third the BER activity of age-matched control mice. The spontaneous mutant frequency for mixed spermatogenic cells prepared from Polb heterozygous mice was 2-fold greater than that of wild-type controls, but no significant effect was found among the somatic tissues tested. These results demonstrate that normal POLB abundance is necessary for normal BER activity, which is critical in maintaining a low germline mutant frequency. Notably, spermatogenic cells respond differently than somatic cells to Polb haploinsufficiency.
ER  -

ALLEN, Diwi; Damon, C. HERBERT; Alex, C. MCMAHAN; Vladimír ROTREKL; Robert, W. SOBOL; Samuel, H. WILSON a Christi, A. WALTER. Mutagenesis Is Elevated in Male Germ Cells Obtained from DNA Polymerase-beta Heterozygous Mice. \textit{Biology of Reproduction}. Madison: Society for the Study of Reproduction, 2008, roč.~79, č.~5, s.~824-831. ISSN~0006-3363.

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