The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae

BÜYÜKGÜZEL, Ender, Pavel HYRŠL a Kemal BÜYÜKGÜZEL. The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae. In SEB Book of abstracts. 2008.

Základní údaje

Originální název

The effects of Eicosanoid biosynthesis inhibitors on oxidative stress, antioxidant response and protein profiles in hemolymph of Galleria mellonella larvae

Název česky

Vliv inhibitorů biosyntézy eicosanoidů na oxidativní stres, antioxidační odpověď a proteinové spektrum hemolymfy larev Galleria mellonella

Autoři

BÜYÜKGÜZEL, Ender (792 Turecko), Pavel HYRŠL (203 Česká republika, garant, domácí) a Kemal BÜYÜKGÜZEL (792 Turecko)

Vydání

SEB Book of abstracts, 2008

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Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30105 Physiology

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/08:00042007

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Galleria mellonella; esculetin; malondialdehyde; glutathione S-transferase; hemolymph proteins

Štítky

esculetin, Galleria mellonella, glutathione S-transferase, hemolymph proteins, malondialdehyde

Příznaky

Mezinárodní význam

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Anotace

V originále

Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in the presence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations caused significant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae. The quantity of the 45 kDa protein fraction from total 16 protein detected was increased with increasing esculetin concentrations. An increase after phenidone and dexamethasone exposure was detected only at 0.001 and 0.01% concentration. Other main hemolymph proteins such as ApoLP-I and storage proteins were not altered in larvae reared on diets amended with the EBIs. We suggest that 45 kDa protein may be an oxidative stress-related protein in hemolymph of G. mellonella. This is the first demonstration that antioxidative responses to a EBI exposure are modulated by a physiological system that includes eicosanoid biosynthesis.

Česky

Effects of eicosanoid biosynthesis inhibitors (EBIs) esculetin, dexamethasone and phenidone on hemolymph concentrations of the oxidative stress indicator malondialdehyde (MDA), antioxidant enzyme (glutathione S-transferase GST) and protein profiles of greater wax moth, Galleria mellonella (L.) seventh instar larvae were investigated. The insects were reared from first instars on artificial diets containing 0.001, 0.01, 0.1 and 1.0 g EBIs per 100 g of diets. MDA content was significantly increased in the presence of esculetin. Low concentrations of esculetin led to increased GST activity. Dexamethasone at concentrations of 0.001 and 0.01% significantly resulted in increased hemolymph MDA content. However, high dietary dexamethasone concentrations caused significant decrease in MDA content while these concentrations resulted in increased GST activity by about three-fold. Dietary phenidone led to increased MDA content and GST activity in the larvae. The quantity of the 45 kDa protein fraction from total 16 protein detected was increased with increasing esculetin concentrations. An increase after phenidone and dexamethasone exposure was detected only at 0.001 and 0.01% concentration. Other main hemolymph proteins such as ApoLP-I and storage proteins were not altered in larvae reared on diets amended with the EBIs. We suggest that 45 kDa protein may be an oxidative stress-related protein in hemolymph of G. mellonella. This is the first demonstration that antioxidative responses to a EBI exposure are modulated by a physiological system that includes eicosanoid biosynthesis.