2008
Perphenazine-Induced block of sodium and transient outward potassium current: experimental and simulation study
BÉBAROVÁ, Markéta; Peter MATEJOVIČ; Michal PÁSEK; Dagmar JANSOVÁ; Milena ŠIMURDOVÁ et. al.Základní údaje
Originální název
Perphenazine-Induced block of sodium and transient outward potassium current: experimental and simulation study
Název česky
Perfenazinem způsobená blokáda sodíkového proudu a přechodného proudu draslíku z buňky: experimentální a simulační studie
Autoři
BÉBAROVÁ, Markéta ORCID; Peter MATEJOVIČ; Michal PÁSEK; Dagmar JANSOVÁ; Milena ŠIMURDOVÁ; Marie NOVÁKOVÁ a Jiří ŠIMURDA
Vydání
Analysis of Biomedical Signals and Images, Brno, Vutium Press, 2008, 1211-412X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30105 Physiology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14110/08:00024258
Organizační jednotka
Lékařská fakulta
UT WoS
000303717200018
Klíčová slova anglicky
sodium current;potassium outward current;simulation
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 19. 1. 2013 22:00, doc. MUDr. Markéta Bébarová, Ph.D.
V originále
The effect of a neuroleptic drug perphenazine on sodium current INa and transient outward potassium current Ito was studied in rat ventricular myocytes at room temperature. Perphenazine reversibly blocked INa (reducing its amplitude; IC50 = 1.24+/-0.10 micromol/l, nH = 0.99+/-0.08) and Ito (accelerating its apparent inactivation with a slight decrease of its amplitude; IC50 = 38.2+/-3.5 micromol/l, nH = 0.75+/-0.07, evaluated from the changes of time integral). Both INa- and Ito-block was use- and frequency-dependent (20%-increase of INablock and 10%-increase of Ito-block under 0.3 and 10 micromol/l perphenazine, respectively, at the stimulation frequency of 3.3 Hz applied after a 15-s rest). The results of quantitative modelling suggest that perphenazine interacts with INa-channels in inactivated states, and with Ito-channels in both open and open-inactivated states.
Česky
The effect of a neuroleptic drug perphenazine on sodium current INa and transient outward potassium current Ito was studied in rat ventricular myocytes at room temperature. Perphenazine reversibly blocked INa (reducing its amplitude; IC50 = 1.24+/-0.10 micromol/l, nH = 0.99+/-0.08) and Ito (accelerating its apparent inactivation with a slight decrease of its amplitude; IC50 = 38.2+/-3.5 micromol/l, nH = 0.75+/-0.07, evaluated from the changes of time integral). Both INa- and Ito-block was use- and frequency-dependent (20%-increase of INablock and 10%-increase of Ito-block under 0.3 and 10 micromol/l perphenazine, respectively, at the stimulation frequency of 3.3 Hz applied after a 15-s rest). The results of quantitative modelling suggest that perphenazine interacts with INa-channels in inactivated states, and with Ito-channels in both open and open-inactivated states.
Návaznosti
| GA305/04/1385, projekt VaV |
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| MSM0021622402, záměr |
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