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@proceedings{778560,
author = {Sedláček, Ivo and Pantůček, Roman and Petráš, Petr and Švec, Pavel},
booktitle = {13th International Symposium on Staphylococci and Staphylococcal Infections},
keywords = {Staphylococcus hominis; automated ribotyping; pulsed field gel electrophoresis},
language = {eng},
title = {Typing of Staphylococcus hominis subsp. novobiosepticus},
url = {http://www.isssi2008.com/},
year = {2008}
}
TY - CONF
ID - 778560
AU - Sedláček, Ivo - Pantůček, Roman - Petráš, Petr - Švec, Pavel
PY - 2008
TI - Typing of Staphylococcus hominis subsp. novobiosepticus
KW - Staphylococcus hominis
KW - automated ribotyping
KW - pulsed field gel electrophoresis
UR - http://www.isssi2008.com/
N2 - Objectives: Staphylococci represent a substantial part of the surface microflora of mammals and birds (skin, skin glands and mucous membranes) and some Staphylococcus species, mainly Staphylococcus aureus subsp. aureus, are found frequently as etiological agents of a variety of human and animal infections. Staphylococcus hominis subsp. novobiosepticus, a pathogen described in the last decade, is unusual novobiocin-resistant and coagulase negative staphylococcus occurring with increasing frequency in human clinical material. The aim of the presented study was to compare the automated ribotyping performed with the RiboPrinter microbial characterization system and pulsed field gel electrophoresis (PFGE) of SmaI macrorestriction fragments for identification and characterization of S. hominis subspecies. Methods: A total of 41 S. hominis cultures comprising two reference type strains and 39 clinical isolates obtained from different microbiological laboratories in the Czech Republic were analysed in this study. All cultures were identified to the subspecies level on the basis of biotyping (STAPHYtest 24, API Staph, conventional tests). Macrorestriction analysis (SmaI) was performed by using a CHEF-DR II system. Automated ribotyping (EcoRI) of 16 representatives of different macrorestriction types selected on the basis of the PFGE results was carried out in accordance with the protocol provided by the manufacturer. Obtained ribotype patterns were processed using the RiboExplorer software and identified using the DuPont qualicon database DUP 2004. Results: Automated ribotyping with EcoRI restriction enzyme generated bands ranging from 1 to 50 kbp and separated 16 analyzed strains into 7 ribogroups. Unfortunately the RiboPrinter system did not assign S. hominis subsp. novobiosepticus isolates to the subspecies level and all strains were misidentified only as S. hominis. Macrorestriction analysis with SmaI restriction enzyme clearly distinguished all 41 cultures at the subspecies level and generated strain-specific fingerprints enabling differentiation of individual strains. Conclusion: Obtained results proved both methods as applicable for S. hominis intrasubspecies typing purposes, however macrorestriction analysis was revealed as more discriminative tool than ribotyping in our study. The automated ribotyping identified analysed strains as S. hominis but the method failed to differentiate the significant pathogen S. hominis subsp. novobiosepticus.
ER -
SEDLÁČEK, Ivo, Roman PANTŮČEK, Petr PETRÁŠ a Pavel ŠVEC. Typing of $<$I$>$Staphylococcus hominis$<$/I$>$ subsp. $<$I$>$novobiosepticus$<$/I$>$. In \textit{13th International Symposium on Staphylococci and Staphylococcal Infections}. 2008.
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