Characteristics of Staphylococcus aureus strains from 5
outbreaks of pemphigus neonatorum in the Czech Republic and
Slovakia

D 2008

Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia

PETRÁŠ, Petr, Ivana MACHOVÁ, Vladislava RŮŽIČKOVÁ a Roman PANTŮČEK

Základní údaje

Originální název

Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia

Název česky

Charakterizace kmenů Staphylococcus aureus z 5 epidemických výskytů pemphigus neonatorum v České republice a na Slovensku

Autoři

PETRÁŠ, Petr (203 Česká republika, garant), Ivana MACHOVÁ (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika) a Roman PANTŮČEK (203 Česká republika)

Vydání

Cairns, Australia, 13th International Symposium on Staphylococci and Staphylococcal Infections, od s. 94-95, 2 s. 2008

Nakladatel

Australian Society for Antimicrobials

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/08:00026432

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus aureus; exfoliative toxin; pemphigus neonatorum; molecular typing; pulsed field gel electrophoresis

Štítky

Exfoliative Toxin, molecular typing, pemphigus neonatorum, pulsed field gel electrophoresis, Staphylococcus aureus

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

Objectives: To characterize S. aureus (SAU) strains producing exfoliative toxins A (ETA) and/or B (ETB) from 5 outbreaks of pemphigus neonatorum. Methods: As many as 366 SAU strains were referred to the National Reference Laboratory for Staphylococci, NIPH, Prague, from pemphigus neonatorum outbreaks in 4 Czech (A, B, C, and D) and 1 Slovak (E) hospitals in 2006 - 2008. The strains were tested by phenotypic (RPLA, phage-typing) and genotypic methods (PFGE; prophage carriage by multiplex PCR targeting prophages significant in lysogenic conversion of ETA production). Results: As many as 118 of the 366 isolates under study were found to produce one, or two of exfoliative toxins. Hospital A. Sixty-nine SAU strains originating from an outbreak persisting for more than 6 months included 12 producers of both ETA and ETB classified into phage type (PT) 3A/3C/55 from newborns and mothers and 8 isolates from health care professionals. Hospital B. Of 35 SAU isolates, 13 were ETA producers (PT 47/75) from newborns and 3 originated from nurses. Hospital C. Altogether 146 SAU strains originated from an extended outbreak (November 2006 through August 2007). Of 36 toxigenic strains, 11 produced ETA and 25 ETA+ETB. The exfoliatin producers showed an identical PFGE pattern. Twenty-nine ETA and/or ETB producers were from newborns (blister fluid, navel swabs, impetigo, paronychia), two originated from mothers (impetigo and nasal swab), and one from a nasal swab from a nurse. The detection of 4 ETA+ETB producers from the hospital environment was of high relevance. The ETA+ETB-positive strains isolated in Hospital C showed identical PFGE patterns as did one strain, ETA+ETB producer, from the Hospital D. Hospital D. Fifty-seven SAU strains from one outbreak of pemphigus neonatorum in 2008. Nineteen strains with hyperproduction of exfoliatin B originated from children and mothers (nasal swabs and impetigo), and one such strain was isolated from children's balm. Slovak Hospital E. A long-standing outbreak has been observed since 2003. Since November 2006, 44 SAU strains were referred to NRL. Fifteen strains were identical ETA producers (PT 47/75) from newborns' blisters and 1 such strain was a nasal isolate from a nurse. Genotyping revealed that the strains isolated in Slovak hospital E in 2003 and 2006 are of the same PFGE type. A highly similar PFGE profile was identified in the causative SAU strains in hospital B. Interestingly, all studied ET-positive strains were harboring the same types of prophages (group B and Fb-like). Conclusions: Genotyping was helpful in identifying the source of infection and controlling nosocomial outbreaks of pemhigus neonatorum in newborns.

Česky

neuvedeno

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

PETRÁŠ, Petr, Ivana MACHOVÁ, Vladislava RŮŽIČKOVÁ a Roman PANTŮČEK. Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia. In 13th International Symposium on Staphylococci and Staphylococcal Infections. Cairns, Australia: Australian Society for Antimicrobials, 2008, s. 94-95.

@inproceedings{778561,
   author = {Petráš, Petr and Machová, Ivana and Růžičková, Vladislava and Pantůček, Roman},
   address = {Cairns, Australia},
   booktitle = {13th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {Staphylococcus aureus; exfoliative toxin; pemphigus neonatorum; molecular typing; pulsed field gel electrophoresis},
   language = {eng},
   location = {Cairns, Australia},
   pages = {94-95},
   publisher = {Australian Society for Antimicrobials},
   title = {Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia},
   url = {http://www.isssi2008.com/},
   year = {2008}
}

TY  - JOUR
ID  - 778561
AU  - Petráš, Petr - Machová, Ivana - Růžičková, Vladislava - Pantůček, Roman
PY  - 2008
TI  - Characteristics of Staphylococcus aureus strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia
PB  - Australian Society for Antimicrobials
CY  - Cairns, Australia
KW  - Staphylococcus aureus
KW  - exfoliative toxin
KW  - pemphigus neonatorum
KW  - molecular typing
KW  - pulsed field gel electrophoresis
UR  - http://www.isssi2008.com/
N2  - Objectives: To characterize S. aureus (SAU) strains producing exfoliative toxins A (ETA) and/or B (ETB) from 5 outbreaks of pemphigus neonatorum. Methods: As many as 366 SAU strains were referred to the National Reference Laboratory for Staphylococci, NIPH, Prague, from pemphigus neonatorum outbreaks in 4 Czech (A, B, C, and D) and 1 Slovak (E) hospitals in 2006 - 2008. The strains were tested by phenotypic (RPLA, phage-typing) and genotypic methods (PFGE; prophage carriage by multiplex PCR targeting prophages significant in lysogenic conversion of ETA production). Results: As many as 118 of the 366 isolates under study were found to produce one, or two of exfoliative toxins. Hospital A. Sixty-nine SAU strains originating from an outbreak persisting for more than 6 months included 12 producers of both ETA and ETB classified into phage type (PT) 3A/3C/55 from newborns and mothers and 8 isolates from health care professionals. Hospital B. Of 35 SAU isolates, 13 were ETA producers (PT 47/75) from newborns and 3 originated from nurses. Hospital C. Altogether 146 SAU strains originated from an extended outbreak (November 2006 through August 2007). Of 36 toxigenic strains, 11 produced ETA and 25 ETA+ETB. The exfoliatin producers showed an identical PFGE pattern. Twenty-nine ETA and/or ETB producers were from newborns (blister fluid, navel swabs, impetigo, paronychia), two originated from mothers (impetigo and nasal swab), and one from a nasal swab from a nurse. The detection of 4 ETA+ETB producers from the hospital environment was of high relevance. The ETA+ETB-positive strains isolated in Hospital C showed identical PFGE patterns as did one strain, ETA+ETB producer, from the Hospital D. Hospital D. Fifty-seven SAU strains from one outbreak of pemphigus neonatorum in 2008. Nineteen strains with hyperproduction of exfoliatin B originated from children and mothers (nasal swabs and impetigo), and one such strain was isolated from children's balm. Slovak Hospital E. A long-standing outbreak has been observed since 2003. Since November 2006, 44 SAU strains were referred to NRL. Fifteen strains were identical ETA producers (PT 47/75) from newborns' blisters and 1 such strain was a nasal isolate from a nurse. Genotyping revealed that the strains isolated in Slovak hospital E in 2003 and 2006 are of the same PFGE type. A highly similar PFGE profile was identified in the causative SAU strains in hospital B. Interestingly, all studied ET-positive strains were harboring the same types of prophages (group B and Fb-like). Conclusions: Genotyping was helpful in identifying the source of infection and controlling nosocomial outbreaks of pemhigus neonatorum in newborns.
ER  -

PETRÁŠ, Petr, Ivana MACHOVÁ, Vladislava RŮŽIČKOVÁ a Roman PANTŮČEK. Characteristics of $<$I$>$Staphylococcus aureus$<$/I$>$ strains from 5 outbreaks of pemphigus neonatorum in the Czech Republic and Slovakia. In \textit{13th International Symposium on Staphylococci and Staphylococcal Infections}. Cairns, Australia: Australian Society for Antimicrobials, 2008, s.~94-95.

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