Changes of IL-6 protein levels in the rat dorsal root ganglia
of two experimental models of neuropathic pain.

a 2008

Changes of IL-6 protein levels in the rat dorsal root ganglia of two experimental models of neuropathic pain.

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Lumír TRENČANSKÝ et al.

Základní údaje

Originální název

Changes of IL-6 protein levels in the rat dorsal root ganglia of two experimental models of neuropathic pain.

Název česky

Změny IL-6 ve spinálních gangliích ve dvouch experimentálních modelech neuropatické bolesti.

Autoři

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Lumír TRENČANSKÝ a Petr DUBOVÝ

Vydání

6th International Symposium on Experimental and Clinical Neurobiology, 2008

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISBN

978-80-7399-489-1

Klíčová slova anglicky

neuropathic pain, peripheral nerve injury, ELISA, cytokines

Změněno: 22. 9. 2008 17:23, Mgr. Zuzana Štumpfová

Anotace

V originále

Cytokines play a role in the induction and maintenance of neuropathic pain. IL-6 is pro-inflammatory cytokine involved in various processes of nervous system including neuronal differentiation, neuroprotection, and neuronal degeneration. Two rat experimental models of neuropathic pain were used for investigation of temporal changes of IL-6 protein levels in both ipsi- and contralateral lumbal (L4-L5) and cervical (C6-C7) dorsal root ganglia (DRG) and plasma. Rats of first experimental model were operated aseptically on triple unilateral ligation of sciatic nerve (ScNL); rats of second experimental model were operated on ligature of L4-L5 spinal nerves (SNL). The animals were divided into three groups: naïve group (rat without any operation, n=6); operated group (n=24) with periods of survival for 1, 3, 7 and 14 days; sham operated group (operated rats without nerve lesion, n=12) with periods of survival 3 and 14 days. Commercially available ELISA test (BioSource, CA, USA) was used for quantitative analysis of IL-6 protein. One day after ScNL, significantly increased levels of IL-6 protein were observed in both ipsilateral and contralateral cervical and lumbal DRG. The other periods of survival displayed just mild increase of IL-6 protein levels close to the control (naïve group) baseline level. Levels of IL-6 protein were significantly increased from post-operation day 1, and rose up to 14 days from SNL operation not only in ipsi- and contralateral L4-L5 DRG, but also in ipsi- and contralateral C6-C7 DRG. In general, elevation of IL-6 protein was greater in lumbal than cervical DRG. Plasma levels of IL-6 protein measured in both models were significantly lower than in DRG tissue and displayed principally the similar pattern. The obtained data provide evidence for changes of IL-6 protein levels not only in the DRG associated with damaged nerve, but also in those non-associated with nerve injury in two rat experimental neuropathic pain models. Stimuli for bilateral increase of IL-6 protein in the DRG after unilateral nervous lesion are probably of systemic character.

Citovat

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Lumír TRENČANSKÝ a Petr DUBOVÝ. Changes of IL-6 protein levels in the rat dorsal root ganglia of two experimental models of neuropathic pain. In 6th International Symposium on Experimental and Clinical Neurobiology. 2008. ISBN 978-80-7399-489-1.

@proceedings{778986,
   author = {Veselková, Zuzana and Klusáková, Ilona and Svíženská, Ivana and Jančálek, Radim and Trenčanský, Lumír and Dubový, Petr},
   booktitle = {6th International Symposium on Experimental and Clinical Neurobiology},
   keywords = {neuropathic pain, peripheral nerve injury, ELISA, cytokines},
   language = {eng},
   isbn = {978-80-7399-489-1},
   title = {Changes of IL-6 protein levels in the rat dorsal root ganglia of two experimental models of neuropathic pain.},
   year = {2008}
}

TY  - CONF
ID  - 778986
AU  - Veselková, Zuzana - Klusáková, Ilona - Svíženská, Ivana - Jančálek, Radim - Trenčanský, Lumír - Dubový, Petr
PY  - 2008
TI  - Changes of IL-6 protein levels in the rat dorsal root ganglia of two experimental models of neuropathic pain.
SN  - 9788073994891
KW  - neuropathic pain, peripheral nerve injury, ELISA, cytokines
N2  - Cytokines play a role in the induction and maintenance of neuropathic pain. IL-6 is pro-inflammatory cytokine involved in various processes of nervous system including neuronal differentiation, neuroprotection, and neuronal degeneration. Two rat experimental models of neuropathic pain were used for investigation of temporal changes of IL-6 protein levels in both ipsi- and contralateral lumbal (L4-L5) and cervical (C6-C7) dorsal root ganglia (DRG) and plasma. Rats of first experimental model were operated aseptically on triple unilateral ligation of sciatic nerve (ScNL); rats of second experimental model were operated on ligature of L4-L5 spinal nerves (SNL). The animals were divided into three groups: naïve group (rat without any operation, n=6); operated group (n=24) with periods of survival for 1, 3, 7 and 14 days; sham operated group (operated rats without nerve lesion, n=12) with periods of survival 3 and 14 days. Commercially available ELISA test (BioSource, CA, USA) was used for quantitative analysis of IL-6 protein. One day after ScNL, significantly increased levels of IL-6 protein were observed in both ipsilateral and contralateral cervical and lumbal DRG. The other periods of survival displayed just mild increase of IL-6 protein levels close to the control (naïve group) baseline level. Levels of IL-6 protein were significantly increased from post-operation day 1, and rose up to 14 days from SNL operation not only in ipsi- and contralateral L4-L5 DRG, but also in ipsi- and contralateral C6-C7 DRG. In general, elevation of IL-6 protein was greater in lumbal than cervical DRG. Plasma levels of IL-6 protein measured in both models were significantly lower than in DRG tissue and displayed principally the similar pattern. The obtained data provide evidence for changes of IL-6 protein levels not only in the DRG associated with damaged nerve, but also in those non-associated with nerve injury in two rat experimental neuropathic pain models. Stimuli for bilateral increase of IL-6 protein in the DRG after unilateral nervous lesion are probably of systemic character.
ER  -

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Lumír TRENČANSKÝ a Petr DUBOVÝ. Changes of IL-6 protein levels in the rat dorsal root ganglia of two experimental models of neuropathic pain. In \textit{6th International Symposium on Experimental and Clinical Neurobiology}. 2008. ISBN~978-80-7399-489-1.

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