Corticosterone administration results in a significant decrease
of IL6r and gp130, but not IL-6 protein in the rat dorsal root
ganglia following sciatic nerve ligature

a 2008

Corticosterone administration results in a significant decrease of IL6r and gp130, but not IL-6 protein in the rat dorsal root ganglia following sciatic nerve ligature

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Petr DUBOVÝ et al.

Základní údaje

Originální název

Corticosterone administration results in a significant decrease of IL6r and gp130, but not IL-6 protein in the rat dorsal root ganglia following sciatic nerve ligature

Autoři

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK a Petr DUBOVÝ

Vydání

6th International Symposium on Experimental and Clinical Neurobiology, 2008

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

ISBN

978-80-7399-489-1

Klíčová slova anglicky

neuropathic pain, immunohistochemistry, IL-6, gp130

Změněno: 23. 9. 2008 15:54, Mgr. Zuzana Štumpfová

Anotace

V originále

Interleukin-6 (IL-6) is proinflammatory cytokine that has been implicated in the development of neuropathic pain. It binds to specific receptor IL-6R and the IL-6/IL-6R complex is responsible for the homodimerization of the transmembranous signal transducer gp130. Many studies have shown that corticosterone administration effectively reduces the production of inflammatory mediators including cytokines. In the present study we detected an immunofluorescence (IF) for IL-6 and its receptors in the rat L4 dorsal root ganglion (DRG) cells of the naïve rats and those following sciatic nerve ligature and corticosterone administrations. Naïve DRG showed only low IF intensity for IL-6 and its receptors in the small- and medium-sized neurons and the satellite glial cells (SGC) surrounding large neurons. Sciatic nerve ligature induced an increased level of IF for IL-6, IL-6R, and gp130 in the small- and medium-sized neurons, and predominantly in the large neuron-SGC units from 3 to 14 days after surgery. While following corticosterone administration 3 and 6 days from operation IF intensity for IL-6 remained at similar level like in DRG without administration, IF intensity for IL-6R and gp130 was markedly decreased, and only a weak IF was found in the neurons. Our results showed that corticosterone administration did not result in expected robust decrease of IF for IL-6 in contrast to IL-6 receptors that mediate a biological effect of IL-6.

Návaznosti

GA309/07/0121, projekt VaV

Název: Změny cytokinů ve spinálních gangliích experimentálních modelů neuropatické bolesti indukované unilaterálním poškozením nervu

Investor: Grantová agentura ČR, Změny cytokinů ve spinálních gangliích experimentálních modelů neuropatické bolesti indukované unilaterálním poškozením nervu

MSM0021622404, záměr

Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Citovat

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK a Petr DUBOVÝ. Corticosterone administration results in a significant decrease of IL6r and gp130, but not IL-6 protein in the rat dorsal root ganglia following sciatic nerve ligature. In 6th International Symposium on Experimental and Clinical Neurobiology. 2008. ISBN 978-80-7399-489-1.

@proceedings{778987,
   author = {Veselková, Zuzana and Klusáková, Ilona and Svíženská, Ivana and Jančálek, Radim and Dubový, Petr},
   booktitle = {6th International Symposium on Experimental and Clinical Neurobiology},
   keywords = {neuropathic pain, immunohistochemistry, IL-6, gp130},
   language = {eng},
   isbn = {978-80-7399-489-1},
   title = {Corticosterone administration results in a significant decrease of IL6r and gp130, but not IL-6 protein in the rat dorsal root ganglia following sciatic nerve ligature},
   year = {2008}
}

TY  - CONF
ID  - 778987
AU  - Veselková, Zuzana - Klusáková, Ilona - Svíženská, Ivana - Jančálek, Radim - Dubový, Petr
PY  - 2008
TI  - Corticosterone administration results in a significant decrease of IL6r and gp130, but not IL-6 protein in the rat dorsal root ganglia following sciatic nerve ligature
SN  - 9788073994891
KW  - neuropathic pain, immunohistochemistry, IL-6, gp130
N2  - Interleukin-6 (IL-6) is proinflammatory cytokine that has been implicated in the development of neuropathic pain. It binds to specific receptor IL-6R and the IL-6/IL-6R complex is responsible for the homodimerization of the transmembranous signal transducer gp130. Many studies have shown that corticosterone administration effectively reduces the production of inflammatory mediators including cytokines. In the present study we detected an immunofluorescence (IF) for IL-6 and its receptors in the rat L4 dorsal root ganglion (DRG) cells of the naïve rats and those following sciatic nerve ligature and corticosterone administrations. Naïve DRG showed only low IF intensity for IL-6 and its receptors in the small- and medium-sized neurons and the satellite glial cells (SGC) surrounding large neurons. Sciatic nerve ligature induced an increased level of IF for IL-6, IL-6R, and gp130 in the small- and medium-sized neurons, and predominantly in the large neuron-SGC units from 3 to 14 days after surgery. While following corticosterone administration 3 and 6 days from operation IF intensity for IL-6 remained at similar level like in DRG without administration, IF intensity for IL-6R and gp130 was markedly decreased, and only a weak IF was found in the neurons. Our results showed that corticosterone administration did not result in expected robust decrease of IF for IL-6 in contrast to IL-6 receptors that mediate a biological effect of IL-6.
ER  -

VESELKOVÁ, Zuzana; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Radim JANČÁLEK a Petr DUBOVÝ. Corticosterone administration results in a significant decrease of IL6r and gp130, but not IL-6 protein in the rat dorsal root ganglia following sciatic nerve ligature. In \textit{6th International Symposium on Experimental and Clinical Neurobiology}. 2008. ISBN~978-80-7399-489-1.

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