Intrapopulation genome size dynamics in Festuca pallens

ŠMARDA, Petr, Petr BUREŠ, Lucie HOROVÁ a Olga ROTREKLOVÁ. Intrapopulation genome size dynamics in Festuca pallens. Annals of Botany. Oxford: Oxford University Press, 2008, roč. 102, č. 4, s. 599-607, 8 s. ISSN 0305-7364.

Základní údaje

• Originální název: Intrapopulation genome size dynamics in Festuca pallens
• Název česky: Vnitropopulační dynamika velikosti genomu u Festuca pallens
• Autoři: ŠMARDA, Petr (203 Česká republika, garant), Petr BUREŠ (203 Česká republika), Lucie HOROVÁ (203 Česká republika) a Olga ROTREKLOVÁ (203 Česká republika).
• Vydání: Annals of Botany, Oxford, Oxford University Press, 2008, 0305-7364.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Velká Británie a Severní Irsko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: odkaz na komentář k článku ve vybraném obsahu časopisu odkaz na článek na stránkách časopisu
• Impakt faktor: Impact factor: 2.755
• Kód RIV: RIV/00216224:14310/08:00024952
• Organizační jednotka: Přírodovědecká fakulta
• UT WoS: 000259326300012
• Klíčová slova anglicky: Nuclear DNA content; intraspecific variation; genome size evolution; heritability; stabilizing selection; grasses; flow cytometry
• Štítky: flow cytometry, genome size evolution, grasses, heritability, intraspecific variation, nuclear DNA content, stabilizing selection
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Background and Aims: It is well known that genome size differs among species. However, information on the variation and dynamics of genome size in wild populations and on the early phase of genome size divergence between taxa is currently lacking. Genome size dynamics, heritability and phenotype effects are analysed here in a wild population of Festuca pallens (Poaceae). Methods: Genome size was measured using flow cytometry with DAPI dye in 562 seedlings from 17 maternal plants varying in genome size. The repeatability of genome size measurements was verified at different seasons through the use of different standards and with propidium iodide dye; the range of variation observed was tested via analysis of double-peaks. Additionally, chromosome counts were made in selected seedlings. Key Results and Conclusions: Analysis of double-peaks showed that genome size varied up to 1.188-fold within all 562 seedlings, 1.119-fold within the progeny of a single maternal plant and 1.117-fold in seedlings from grains of a single inflorescence. Generally, genome sizes of seedlings and their mothers were highly correlated. However, in maternal plants with both larger and smaller genomes, genome sizes of seedlings were shifted towards the population median. This was probably due to the frequency of available paternal genomes (pollen grains) in the population. There was a stabilizing selection on genome size during the development of seedlings into adults, which may be important for stabilizing genome size within species. Furthermore, a positive correlation was found between genome size and the development rate of seedlings. A larger genome may therefore provide a competitive advantage, perhaps explaining the higher proportion of plants with larger genomes in the population studied. The reason for the observed variation may be the recent induction of genome size variation, e.g. by activity of retrotransposons, which may be preserved in the long term by the segregation of homeologous chromosomes of different sizes during gametogenesis.

Anotace česky

Background and Aims: It is well known that genome size differs among species. However, information on the variation and dynamics of genome size in wild populations and on the early phase of genome size divergence between taxa is currently lacking. Genome size dynamics, heritability and phenotype effects are analysed here in a wild population of Festuca pallens (Poaceae). Methods: Genome size was measured using flow cytometry with DAPI dye in 562 seedlings from 17 maternal plants varying in genome size. The repeatability of genome size measurements was verified at different seasons through the use of different standards and with propidium iodide dye; the range of variation observed was tested via analysis of double-peaks. Additionally, chromosome counts were made in selected seedlings. Key Results and Conclusions: Analysis of double-peaks showed that genome size varied up to 1.188-fold within all 562 seedlings, 1.119-fold within the progeny of a single maternal plant and 1.117-fold in seedlings from grains of a single inflorescence. Generally, genome sizes of seedlings and their mothers were highly correlated. However, in maternal plants with both larger and smaller genomes, genome sizes of seedlings were shifted towards the population median. This was probably due to the frequency of available paternal genomes (pollen grains) in the population. There was a stabilizing selection on genome size during the development of seedlings into adults, which may be important for stabilizing genome size within species. Furthermore, a positive correlation was found between genome size and the development rate of seedlings. A larger genome may therefore provide a competitive advantage, perhaps explaining the higher proportion of plants with larger genomes in the population studied. The reason for the observed variation may be the recent induction of genome size variation, e.g. by activity of retrotransposons, which may be preserved in the long term by the segregation of homeologous chromosomes of different sizes during gametogenesis.

Návaznosti

• GP206/08/P222, projekt VaV: Název: Evoluce GC obsahu a genomu trav

Investor: Grantová agentura ČR, Evoluce GC obsahu a genomu trav

• LC06073, projekt VaV: Název: Centrum pro výzkum biodiverzity

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum biodiverzity

• MSM0021622416, záměr: Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase