| POHANKA, Miroslav, Petr SKLÁDAL a Ota PAVLIŠ. A label free piezoelectric immunosensor for rapid assay of Escherichia coli. Journal of Immunoassay and Immunochemistry. Abingdon, Velká Británie: Taylor and Francis, 2008, roč. 29, č. 1, s. 70-79. ISSN 1532-1819. |
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@article{788776,
author = {Pohanka, Miroslav and Skládal, Petr and Pavliš, Ota},
article_location = {Abingdon, Velká Británie},
article_number = {1},
keywords = {Piezoelectric biosensor; Quartz crystal microbalance; Immunoassay; Immunosensor; Escherichia coli},
language = {eng},
issn = {1532-1819},
journal = {Journal of Immunoassay and Immunochemistry},
note = {DOI: 10.1080/15321810701735120 IF 2007: 0.614},
title = {A label free piezoelectric immunosensor for rapid assay of Escherichia coli},
volume = {29},
year = {2008}
}
TY - JOUR
ID - 788776
AU - Pohanka, Miroslav - Skládal, Petr - Pavliš, Ota
PY - 2008
TI - A label free piezoelectric immunosensor for rapid assay of Escherichia coli
JF - Journal of Immunoassay and Immunochemistry
VL - 29
IS - 1
SP - 70-79
EP - 70-79
PB - Taylor and Francis
SN - 15321819
N1 - DOI: 10.1080/15321810701735120 IF 2007: 0.614
KW - Piezoelectric biosensor
KW - Quartz crystal microbalance
KW - Immunoassay
KW - Immunosensor
KW - Escherichia coli
N2 - piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 106-109 CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 108 CFU/mL.
ER -
POHANKA, Miroslav, Petr SKLÁDAL a Ota PAVLIŠ. A label free piezoelectric immunosensor for rapid assay of Escherichia coli. \textit{Journal of Immunoassay and Immunochemistry}. Abingdon, Velká Británie: Taylor and Francis, 2008, roč.~29, č.~1, s.~70-79. ISSN~1532-1819.
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