| MOLINKOVÁ, Dana, Petr SKLÁDAL a Vladimír CELER. In vitro neutralization of Equid herpesvirus 1 mediated by recombinant antibodies. Journal of Immunological Methods. Amsterdam, Holandsko: Elsevier, 2008, roč. 333, č. 1, s. 186-191. ISSN 0022-1759. |
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@article{788778,
author = {Molinková, Dana and Skládal, Petr and Celer, Vladimír},
article_location = {Amsterdam, Holandsko},
article_number = {1},
keywords = {Neutralization; Antibody fragments; scFv; Glycoprotein},
language = {eng},
issn = {0022-1759},
journal = {Journal of Immunological Methods},
note = {doi:10.1016/j.jim.2008.01.019 IF 2007: 1.947},
title = {In vitro neutralization of Equid herpesvirus 1 mediated by recombinant antibodies},
volume = {333},
year = {2008}
}
TY - JOUR
ID - 788778
AU - Molinková, Dana - Skládal, Petr - Celer, Vladimír
PY - 2008
TI - In vitro neutralization of Equid herpesvirus 1 mediated by recombinant antibodies
JF - Journal of Immunological Methods
VL - 333
IS - 1
SP - 186-191
EP - 186-191
PB - Elsevier
SN - 00221759
N1 - doi:10.1016/j.jim.2008.01.019 IF 2007: 1.947
KW - Neutralization
KW - Antibody fragments
KW - scFv
KW - Glycoprotein
N2 - A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
ER -
MOLINKOVÁ, Dana, Petr SKLÁDAL a Vladimír CELER. In vitro neutralization of Equid herpesvirus 1 mediated by recombinant antibodies. \textit{Journal of Immunological Methods}. Amsterdam, Holandsko: Elsevier, 2008, roč.~333, č.~1, s.~186-191. ISSN~0022-1759.
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