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@article{791483,
author = {Fabrik, Ivo and Ruferová, Zuzana and Hilscherová, Klára and Adam, Vojtěch and Trnková, Libuše and Kizek, René},
article_location = {Basel Switzerland},
article_number = {7},
keywords = {electrochemistry; heavy metals;metallothionein},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction},
volume = {8},
year = {2008}
}
TY - JOUR
ID - 791483
AU - Fabrik, Ivo - Ruferová, Zuzana - Hilscherová, Klára - Adam, Vojtěch - Trnková, Libuše - Kizek, René
PY - 2008
TI - A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction
JF - Sensors
VL - 8
IS - 7
SP - 4081-4094
EP - 4081-4094
PB - MOLECULAR DIVERSITY PRESERVATION INT
SN - 14248220
KW - electrochemistry
KW - heavy metals;metallothionein
N2 - Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationary electrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM. Moreover at field study chironomid larvae as well as sediment samples have been collected from eight field sites with different levels of pollution by heavy. The metals content (chromium, nickel, copper, zinc, arsenic, molybdenum,cadmium, tin and lead) in the sediment and or MT content in the chironomid larvae were determined by inductively coupled plasma mass spectrometry or Brdicka reaction, respectively.
ER -
FABRIK, Ivo, Zuzana RUFEROVÁ, Klára HILSCHEROVÁ, Vojtěch ADAM, Libuše TRNKOVÁ a René KIZEK. A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction. \textit{Sensors}. Basel Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč.~8, č.~7, s.~4081-4094. ISSN~1424-8220.
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