| ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ a René KIZEK. An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes. Sensors. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč. 8, č. 4, s. 2293-2305, 12 s. ISSN 1424-8220. |
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@article{791493,
author = {Adam, Vojtěch and Baloun, Jiří and Fabrik, Ivo and Trnková, Libuše and Kizek, René},
article_location = {Basel, Switzerland},
article_number = {4},
keywords = {metallothionein; voltammetry; biosensor},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes},
volume = {8},
year = {2008}
}
TY - JOUR
ID - 791493
AU - Adam, Vojtěch - Baloun, Jiří - Fabrik, Ivo - Trnková, Libuše - Kizek, René
PY - 2008
TI - An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes
JF - Sensors
VL - 8
IS - 4
SP - 2293-2305
EP - 2293-2305
PB - MOLECULAR DIVERSITY PRESERVATION INT
SN - 14248220
KW - metallothionein
KW - voltammetry
KW - biosensor
N2 - We report on improvement of the adsorptive transfer stripping technique (AdTS) coupled with the differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current technique has been unable to generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying the current transfer technique including cooling step of the adsorbed analyte. We tested the technique on determination of a promising tumour disease marker protein called metallothionein (MT). The detection limit (3 S/N) of MT was evaluated as 500 zeptomoles per 500 nL (1 pM) and the quantification limit (10 S/N) as 1,500 zeptomoles per 500 nL (3 pM). Further, the improved AdTS technique was utilized to analyze blood serum samples from patients with breast cancer. Based on the results obtained it can be concluded that the improved technique can be used to detect a thiolprotein in very low sample volumes and can also prevent interferences during the washing and transferring step.
ER -
ADAM, Vojtěch, Jiří BALOUN, Ivo FABRIK, Libuše TRNKOVÁ a René KIZEK. An electrochemical detection of metallothioneins at the zeptomole level in nanolitre volumes. \textit{Sensors}. Basel, Switzerland: MOLECULAR DIVERSITY PRESERVATION INT, 2008, roč.~8, č.~4, s.~2293-2305, 12 s. ISSN~1424-8220.
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