Constant current chronopotentiometry and voltammetry of native and denatured serum albumin at mercury and carbon electrodes

OSTATNÁ, Veronika, Filiz KURALAY, Libuše TRNKOVÁ and Emil PALEČEK. Constant current chronopotentiometry and voltammetry of native and denatured serum albumin at mercury and carbon electrodes. Electroanalysis. Weinheim, Germany: Wiley-VCH Verlag GmbH, 2008, vol. 20, No 13, p. 1406-1413. ISSN 1040-0397.

Basic information

• Original name: Constant current chronopotentiometry and voltammetry of native and denatured serum albumin at mercury and carbon electrodes
• Name in Czech: Chronopotenciometrie a voltametrie nativního a denaturovaného sérového albuminu na rtuťových a uhlíkových elektrodách
• Authors: OSTATNÁ, Veronika (703 Slovakia), Filiz KURALAY (792 Turkey), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and Emil PALEČEK (203 Czech Republic).
• Edition: Electroanalysis, Weinheim, Germany, Wiley-VCH Verlag GmbH, 2008, 1040-0397.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10405 Electrochemistry
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.901
• RIV identification code: RIV/00216224:14310/08:00034074
• Organization unit: Faculty of Science
• UT WoS: 000257618300003
• Keywords in English: chronopotentiometry; voltammetry; albumin
• Tags: albumin, chronopotentiometry, Voltammetry

Abstract

Constant current chronopotentiometric peak H at mercury electrodes was recently shown as a sensitive tool for global and local changes in protein conformation. Large differences between the heights of peak H of native (hBSAnat) and denatured BSA (hBSAden) were observed. The ratio hBSAden/hBSAnat increased with more negative stripping current suggesting that the rate of potential change is important for discrimination between native and denatured BSA. Voltammetric peaks of BSA were less well developed and BSAden/BSAnat was much smaller. It was not possible to discriminate BSAden and BSAnat using carbon electrodes.

Abstract (in Czech)

Chronopotenciometrický pík H na rtuťové elektrodě se nedávno ukázal jako citlivý nástroj pro studium globálních i lokálních změn v konformaci proteinů. Byly pozorovány velké rozdíly ve výšce píku nativního a denaturovaného albuminu.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• OC 174, research and development project: Name: Využití rozšířených elektrochemických metod ke studiu elektrochemických a bioelektrochemických procesů na mezifázi elektroda/elektrolyt

Investor: Ministry of Education, Youth and Sports of the CR, Utilization of Hyphenated Electrochemical Methods to the Study of Electrochemical and Bio-electrochemical Processes at Solid-Electrolyte Interfaces